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SAB4200451

Sigma-Aldrich

Anti-NMD3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-60S Ribosomal export protein NMD3, Anti-CGI-07, Anti-NMD3 homolog (S. cerevisiae)
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

human, rat, mouse

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells.
western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells over-expressing mouse NMD3.

UniProt accession no.

Q96D46

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NMD3(51068)
mouse ... Nmd3(97112)
rat ... Nmd3(310512)

General description

NMD3 ribosome export adaptor (NMD3) is an adaptor for 60S subunit export via the export receptor CRM1 (XPO1). In human, the C-terminal domain of NMD3 comprises a CRM1- dependent leucine-rich nuclear export signal (NES) and a nuclear localization signal (NLS) in its. Two zinc-binding centres and the ribosome-binding domain forms the N-terminal domain.

Immunogen

peptide corresponding to an internal region of human NMD3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and dog.

Application

Anti-NMD3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

NMD3 ribosome export adaptor (NMD3) is a highly conserved nucleocytoplasmic shuttling protein, essential for nuclear export of 60S ribosomal subunits.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates
Trotta CR, et al.
The Embo Journal, 22(11), 2841-2851 (2003)
Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit
Sengupta J, et al.
The Journal of Cell Biology, 189(7), 1079-1086 (2010)
Baoyan Bai et al.
Nucleus (Austin, Tex.), 4(4), 315-325 (2013-06-21)
CRM1 is an export factor that together with its adaptor NMD3 transports numerous cargo molecules from the nucleus to cytoplasm through the nuclear pore. Previous studies have suggested that CRM1 and NMD3 are detected in the nucleolus. However, their localization

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