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SAB4200434

Sigma-Aldrich

Anti-VHL antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-HRCA1, Anti-RCA1, Anti-VHL1, Anti-Von Hippel-Lindau tumor supressor
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~30 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells overexpressing human VHL
indirect immunofluorescence: 5-10 μg/mL using HeLa cells
western blot: 1.5-3.0 μg/mL using lysates of HEK-293T cells overexpressing human VHL

UniProt accession no.

P40337

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VHL(7428)

General description

Von Hippel-Lindau tumor suppressor (VHL) is encoded by the gene mapped to human chromosome 3p25. The encoded protein is mainly expressed in adult brain and kidney. It is specifically localized on the cell membrane.

Immunogen

synthetic peptide corresponding to a sequence near the C-terminus of human VHL, conjugated to KLH. The corresponding sequence is identical in human VHL isoform 2 and highly conserved (94% sequence identity) in rat VHL.

Application

Anti-VHL antibody produced in rabbit has been used in immunoblotting, immunoprecipitation and immunofluorescence.

Biochem/physiol Actions

VHL acts as part of an E3 ubiquitin ligase that targets hypoxia-inducible factor α (HIF α) for rapid proteasomal degradation under normoxic conditions. VHL has been also identified as a key regulator of microtubule dynamic stability.
Von Hippel-Lindau tumor suppressor (VHL) acts as a tumor suppressor in some cancers. It might also play a vital role in signal transduction or cell adhesion. Mutation in the gene is associated with the development of sporadic cerebellar hemangioblastoma tumorigenesis and von Hippel-Lindau (VHL) disease. Interaction of the protein with hypoxia-inducible factor-1 (HIF-1) induces mammalian oxygen sensing.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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The Clinicopathological Significance of Epigenetic Silencing of VHL Promoter and Renal Cell Carcinoma: A Meta-Analysis.
Yang L
Cellular Physiology and Biochemistry, 40, 1465-1472 (2016)
The VHL tumor suppressor and HIF: insights from genetic studies in mice
Kapitsinou PP and Haase VH
Cell Death and Differentiation, 15(4), 650-650 (2008)
The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
Maxwell PH
Nature, 399, 271-275 (1999)
Loss of Heterozygosity and Somatic Mutations of the VHL Tumor Suppressor Gene in Sporadic Cerebellar Hemangioblastomas1
Lee JY
Cancer Research, 58, 504-508 (1998)
HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing.
Ivan M
Science, 292, 464-468 (2001)
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