SAB4200415
Anti-NEZHA antibody, Mouse monoclonal
clone NEZHA-1, purified from hybridoma cell culture
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Synonym(s):
Anti-CAMSAP3, Anti-Calmodulin regulated spectrin-associated protein family member 3, Anti-KIAA, Monoclonal Anti-NEZHA antibody produced in mouse
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NEZHA-1, monoclonal
form
buffered aqueous solution
mol wt
antigen ~150 kDa
species reactivity
canine, human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CAMSAP3(57662)
mouse ... Camsap3(69697)
Related Categories
General description
Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13.
NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA.
Application
Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining.
Monoclonal Anti-NEZHA antibody produced in mouse has been used in:
- immunofluorescence
- immunoprecipitation
- immunofluorescence
- mass-spectrometry-based analysis of a streptavidin pull-down assay
- immunostaining
Biochem/physiol Actions
CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon.
NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling
Kourtidis A, et al.
The Journal of cell biology, 216(10), 3073-3085 (2017)
CAMSAP3-dependent microtubule dynamics regulates Golgi assembly in epithelial cells.
Wang J
Journal of Genetics and Genomics = Yi Chuan Xue Bao, 44, 39-49 (2017)
CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells.
Toya M
Proceedings of the National Academy of Sciences of the USA, 113, 332-337 (2016)
Laure Coquand et al.
The Journal of cell biology, 220(8) (2021-05-22)
Neurons of the neocortex are generated by stem cells called radial glial cells. These polarized cells extend a short apical process toward the ventricular surface and a long basal fiber that acts as a scaffold for neuronal migration. How the
CAMSAP3 maintains neuronal polarity through regulation of microtubule stability
Pongrakhananon V, et al.
Proceedings of the National Academy of Sciences of the USA, 115(39), 9750-9755 (2018)
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