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SAB4200366

Sigma-Aldrich

Anti-IKZF1/IKAROS antibody, Mouse monoclonal

clone E1.14, purified from hybridoma cell culture

Synonym(s):

Anti-Hs.54452, Anti-IK1, Anti-IKAROS family zinc finger 1, Anti-LYF1, Anti-PRO0758, Anti-ZNFN1A1, Anti-hIk-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

E1.14, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, monkey, human, mouse

concentration

~1.0 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: 0.25-0.5 μg/mL using Jurkat cells
indirect immunofluorescence: suitable
western blot: 0.25-0.5 μg/mL using Ramos cells

isotype

IgG2b

UniProt accession no.

Q13422

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IKZF1(10320)

Related Categories

General description

Monoclonal Anti- IKZF1 (Ikaros family zinc finger protein 1)/IKAROS (mouse IgG2b isotype) is derived from the hybridoma E1.14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide located at the C-terminus human IKZF1/IKAROS. The IKAROS gene encodes a family of hemopoietic specific zinc finger transcriptional regulator proteins, which binds to specific DNA target sequences consisting of TGGGAA consensus motif.

Immunogen

a peptide located at the C-terminus human IKZF1/IKAROS.

Application

Monoclonal Anti-IKZF1 (Ikaros family zinc finger protein 1)/IKAROS antibody produced in mouse has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • flow cytometry

Biochem/physiol Actions

Ikaros family zinc finger protein 1 (IKZF1)/IKAROS activity is essential for the generation of B cell precursors. Ikaros is essential at the earliest stage of T cell and B cell specification. In adults, however, lymphoid lineages rely on Ikaros at distinct phases of their development. Mutations or polymorphisms are associated with human B-cell acute lymphoblastic leukemia (B-ALL). Ikaros functions as a tumor suppressor in T-cell acute lymphoblastic leukemia (T-ALL) and B-ALL. Ikaros deletion was detected in patients with blast-phase myeloproliferative neoplasms.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ikaros is critical for B cell differentiation and function
Kirstetter P, et al.
European Journal of Immunology, 32(3), 720-730 (2002)
Novel mutations and their functional and clinical relevance in myeloproliferative neoplasms: JAK2, MPL, TET2, ASXL1, CBL, IDH and IKZF1
Tefferi A
Leukemia, 24(6), 1128-1128 (2010)
Role of Ikaros in T-cell acute lymphoblastic leukemia
Kastner P and Chan S
World Journal of Biological Chemistry, 2(6), 108-108 (2011)

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