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Merck
CN

SAB4200365

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-PIWIL1 antibody, Mouse monoclonal, clone PIWIL1-2, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PIWIL1-2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

isotype

IgG2b

UniProt accession no.

Q96J94

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • flow cytometry analysis29}
  • immunoblotting
  • immunohistochemistry

Biochem/physiol Actions

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

Immunogen

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000502705
0000502705
0000304839
0000304839
0000253725

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Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
Qiao D, et al.
Oncogene, 21(25), 3988-3988 (2002)
An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes
He X, et al.
Nucleic Acids Research, 43(7), 3712-3725 (2015)
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens
Baumann B, et al.
Prostate, 79(8), 840-855 (2019)
The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, 25, 355-376 (2009)
Xue Zhang et al.
Journal of immunology (Baltimore, Md. : 1950), 196(4), 1591-1603 (2016-01-13)
The discovery of PIWI-interacting RNAs (piRNAs) revealed the complexity of the RNA world. Although piRNAs were first deemed to be germline specific, substantial evidence shows their various roles in somatic cells; however, their function in highly differentiated immune cells remains
View All Related Papers

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