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SAB4200361

Sigma-Aldrich

Anti-LC3B antibody, Mouse monoclonal

clone LC3B-6, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-ATG8F, Monoclonal Anti-LC3, Monoclonal Anti-MAP1A/1BLC3, Monoclonal Anti-MAP1LC3B, Monoclonal Anti-microtubule-associated protein 1 light chain 3 beta

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LC3B-6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~18/16 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells.
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B.

isotype

IgG2b

UniProt accession no.

Q9GZQ8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1LC3B(81631)

Related Categories

General description

The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. Anti-LC3B antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma LC3B-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human LC3B.

Immunogen

synthetic peptide corresponding to the N-terminal region of human LC3B, conjugated to KLH. The corresponding sequence differs by one amino-acid in rat and mouse.

Application

Anti-LC3B antibody produced in rabbit has been used in:
  • western blotting
  • immunofluorescence
  • confocal laser microscopy
  • immunoprecipitation
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Weijie Dong et al.
Autophagy, 14(10), 1677-1692 (2018-07-03)
Microvascular barrier dysfunction is the central pathophysiological feature of acute lung injury (ALI). RAB26 is a newly identified small GTPase involved in the regulation of endothelial cell (EC) permeability. However, the mechanism behind this protection has not been clearly elucidated.
Jean-Baptiste Gorin et al.
Frontiers in medicine, 2, 74-74 (2015-11-06)
Radiation emitted by the radionuclides in radioimmunotherapy (RIT) approaches induce direct killing of the targeted cells as well as indirect killing through the bystander effect. Our research group is dedicated to the development of α-RIT, i.e., RIT using α-particles especially
Xing Feng et al.
Autophagy, 1-20 (2020-03-19)
Although the treatment of brain tumors by targeting kinase-regulated macroautophagy/autophagy, is under investigation, the precise mechanism underlying autophagy initiation and its significance in glioblastoma (GBM) remains to be defined. Here, we report that PAK1 (p21 [RAC1] activated kinase 1) is
Shu Zhang et al.
Cell death & disease, 8(2), e2586-e2586 (2017-02-06)
EVA1A (Eva-1 homologue A) is a novel lysosome and endoplasmic reticulum-associated protein that can regulate cell autophagy and apoptosis. Eva1a is expressed in the myocardium, but its function in myocytes has not yet been investigated. Therefore, we generated inducible, cardiomyocyte-specific
Autophagic degradation of the inhibitory p53 isoform Delta133p53alpha as a regulatory mechanism for p53-mediated senescence
Horikawa I, et al.
Nature Communications, 5 (2014)
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