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SAB4200334

Sigma-Aldrich

Anti-phospho-CD3ζ (pTyr142) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Synonym(s):
Anti-CD247, Anti-CD3H, Anti-CD3Q, Anti-CD3Z, Anti-T cell receptor T3 zeta chain, Anti-T3Z, Anti-TCRK, Anti-Tcrz
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.

UniProt accession no.

P24161

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr142)

Gene Information

human ... CD247(919)
mouse ... Cd247(12503)

Related Categories

General description

The TCR-CD3 multimeric complex is composed of TCRαβ, CD3γε and CD3δε and TCR‐ζ homodimer. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.

Immunogen

synthetic phosphopeptide corresponding to residues surrounding Tyr142 of mouse CD3ζ, conjugated to KLH. The corresponding sequence is identical in human CD3ζ.

Application

Anti-phospho-CD3ζ (pTyr142) antibody produced in rabbit has been used in several immunochemical techniques including immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Amongst the TCR subunits, the CD3 ζ chain plays a key role in regulating TCR assembly, transport, and cell surface expression. Down-regulation of TCR ζ chain expression associated with T cell dysfunction was described in various pathologies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment
Kim K D, et al.
Nature Communications, 8, 1-10 (2017)
TCR zeta-chain downregulation: curtailing an excessive inflammatory immune response
Baniyash M.
Nature Reviews: Immunology, 4, 675-687 (2004)
Kyun-Do Kim et al.
Nature communications, 8, 15365-15365 (2017-05-16)
Adoptive cell transfer utilizing tumour-targeting cytotoxic T lymphocytes (CTLs) is one of the most effective immunotherapies against haematological malignancies, but significant clinical success has not yet been achieved in solid tumours due in part to the strong immunosuppressive tumour microenvironment.
An architectural perspective on signaling by the pre?, ?? and ?? T cell receptors
Hayes SM, et al.
Immunological Reviews, 191, 28-37 (2003)
Function of Cd3?-Mediated Signals in T Cell Development
Sommers C. L.
The Journal of Experimental Medicine, 192, 913-913 (2000)

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