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SAB4200287

Sigma-Aldrich

Anti-Actin, Muscle specific antibody, Mouse monoclonal

clone HHF-35, purified from hybridoma cell culture

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HHF-35, monoclonal

form

buffered aqueous solution

mol wt

antigen ~42 kDa

species reactivity

rat, bovine, canine, chicken, human, mouse, hamster, monkey

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: 2-4 μg/mL using A10 cells
immunohistochemistry: suitable
western blot: 0.1-0.2 μg/mL using A10 cell extracts

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)

Related Categories

General description

Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.

Immunogen

SDS-extracted protein fraction of human myocardium.

Application

The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.

Biochem/physiol Actions

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Live cell imaging of the cytoskeleton
Test, 505, 203-217 (2012)
Jose Luis Martin-Ventura et al.
Atherosclerosis, 198(2), 264-271 (2008-04-02)
Parathyroid hormone-related protein (PTHrP) is overexpressed in atherosclerotic plaques by unknown mechanisms. We have examined here the putative mechanism(s) responsible for this overexpression in the atherosclerotic lesion and its potential modulation by simvastatin, both in vitro and in vivo. Atherosclerosis
T Tsukada et al.
The American journal of pathology, 126(1), 51-60 (1987-01-01)
A monoclonal antibody to muscle cell actin isotypes was produced and characterized. Immunocytochemical analysis of methanol-Carnoy's-fixed, paraffin-embedded human tissue revealed that this antibody, termed HHF35, reacts with skeletal muscle cells, cardiac muscle cells, smooth muscle cells, pericytes, and myoepithelial cells
Actin structure and function
Dominguez R, et al.
Annual Review of Biophysics, 40, 169-186 (2011)

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