SAB4200256
Anti-AKT1S1 antibody, Mouse monoclonal
clone AKT1S1-3, purified from hybridoma cell culture
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Synonym(s):
Monoclonal Anti-AKT1 substrate 1 (proline-rich), Monoclonal Anti-Lobe, Monoclonal Anti-PRAS40, Monoclonal Anti-PROLINE-RICH AKT SUBSTRATE, 40-KD
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
AKT1S1-3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~40 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AKT1S1(84335)
General description
AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa).
Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1.
Immunogen
The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1.
fusion protein corresponding to the N-terminus of human AKT1S1.
Application
Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation.
Biochem/physiol Actions
Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Role of PRAS40 in Akt and mTOR signaling in health and disease
Wiza C, et al.
American Journal of Physiology. Endocrinology and Metabolism, 302(12), E1453-E1460 (2012)
PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding
Wang L, et al.
Test, 282(27), 20036-20044 (2007)
PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex
Fonseca BD, et al.
Test, 282(34), 24514-24524 (2007)
Emilie Vander Haar et al.
Nature cell biology, 9(3), 316-323 (2007-02-06)
Insulin stimulates protein synthesis and cell growth by activation of the protein kinases Akt (also known as protein kinase B, PKB) and mammalian target of rapamycin (mTOR). It was reported that Akt activates mTOR by phosphorylation and inhibition of tuberous
Bei Huang et al.
Acta pharmacologica Sinica, 26(10), 1253-1258 (2005-09-22)
To study the expression of proline-rich Akt-substrate PRAS40 in the cell survival pathway and tumor progression. The effects of three key kinase inhibitors on PRAS40 activity in the cell survival pathway, serum withdrawal, H(2)O(2) and overexpression of Akt were tested.
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