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SAB4200215

Sigma-Aldrich

Anti-VPS4A antibody, Mouse monoclonal

clone VPS4-110, purified from hybridoma cell culture

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Synonym(s):
Anti-SKD1, SKD1A, SKD2, VPS4-1, Anti-Vacuolar protein sorting factor 4A, Anti-hVPS4
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VPS4-110, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

mouse, canine, bovine, human, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or human HEK-293 cells.

isotype

IgG1

UniProt accession no.

Q9UN37

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VPS4A(27183)
mouse ... Vps4a(116733)
rat ... Vps4a(246772)

General description

Monoclonal Anti-VPS4A (mouse IgG1 isotype) is derived from the hybridoma VPS4-110 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human VPS4A, conjugated to KLH.
Vacuolar-protein-sorting factor 4A (VPS4A) is an AAAtype ATPase, encoded by the gene mapped to human chromosome 16q22.1. The encoded protein is a member of vacuolar sorting protein family. The protein is characterized by a canonical AAA ATPase cassette followed by an N-terminal MIT (microtubule interacting and trafficking) domain.

Application

Monoclonal Anti-VPS4A antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunolabelling

Biochem/physiol Actions

Vacuolar-protein-sorting factor 4A (VPS4A) plays a vital role in lysosomal/endosomal membrane trafficking. It is also involved in the regulation of exosome biogenesis. Downregulated expression of the gene leads to the development of hepatocellular carcinoma (HCC). Thus, VPS4 is considered to be an effective tumor suppressor for HCC. Mutation of the gene leads to defective multivesicular body formation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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A fourth locus for autosomal dominant hypercholesterolemia maps at 16q22.1
Marques-Pinheiro A
European Journal of Human Genetics, 18, 1236-1242 (2010)
Comparative sequence and expression analyses of four mammalian VPS4 genes
Beyer A
Gene, 305, 47-59 (2003)
Disruption of Neurexin 1 Associated with Autism Spectrum Disorder
Kim HG
American Journal of Human Genetics, 82, 199-207 (2008)
Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A
Scott A
Proceedings of the National Academy of Sciences of the USA, 102, 13813-13818 (2005)
Triggered recruitment of ESCRT machinery promotes endolysosomal repair
Skowyra M L, et al.
Science, 360(6384), eaar5078-eaar5078 (2018)
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