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SAB4200211

Sigma-Aldrich

Anti-Phospholipase A2 (cPLA2) (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-PLA2G4, Anti-PLA2G4A, Anti-cPLA2-alpha, Anti-cytosolic phospholipase A2, group IVA

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About This Item

MDL number:
MFCD02686076
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~100 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 15-30 μg using HEK-293T cells over expressing human cPLA2
western blot: 1.5-3 μg/mL using extracts of HEK-293T cells over expressing human cPLA2

UniProt accession no.

P47712

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PLA2G4A(5321)

General description

Download the CofA, SDS and IFU specific to your product lot and format
Fragment mixture prepared by cleavage of λDNA with Hind III.
The product is also available as DNA Molecular Weight Marker II, Digoxigenin-labeled.
Size Range: 0.12 to 23.1 kbp
Contents
Ready-to-use solution, 250 μg/mL, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0)
CPLA2/PLA2G4A (phospholipase A2, group IVA(cytosolic, calcium-dependent)) is located on human chromosome 1q31.1. cPLA2α is an 85 kDa protein made of 749 amino acids. It is a member of the the α-β hydrolase family. CPLA2 has an N-terminal C2 domain (Ca2+ - dependent, phospholipid binding) and two catalytic domains (α/β hydrolase). This protein is located in the cytoplasm.

Specificity

Anti-Phospholipase A2 (cPLA2) (N-terminal), specifically recognizes human cPLA2.

Application

Anti-Phospholipase A2 (cPLA2) (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

CPLA2/PLA2G4A (phospholipase A2, group IVA(cytosolic, calcium-dependent)) helps to hydrolyze phospholipids at the sn-2-acyl ester bond to generate free fatty acid and lysophospholipid second messengers. It is associated with several diseases like cardiovascular disease, diabetes, asthma and arthritis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at −20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
080M4826

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PLA2G4A (phospholipase A2, group IVA (cytosolic, calcium-dependent))
Linkous A and Yazlovitskaya E
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2010)
Sangita Paul et al.
eNeuro, 9(6) (2022-11-10)
Hyperactivation of cyclin-dependent kinase 5 (Cdk5) by p25, contributes to neuroinflammation causing neurodegeneration in Parkinson's disease (PD) and Alzheimer's disease. However, the mechanism by which Cdk5 induces neuroinflammation in the PD brain is largely unexplored. Here, we show that Cdk5

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