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SAB4200203

Sigma-Aldrich

Anti-RAVER1 antibody, Mouse monoclonal

clone RAV1, purified from hybridoma cell culture

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Synonym(s):
Anti-Ribonucleoprotein PTB-binding 1
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RAV1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human, monkey

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1.0-2.0 μg/mL using HeLa or HepG2 cells extract

isotype

IgG1

UniProt accession no.

Q8IY67

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAVER1(125950)

General description

Monoclonal Anti-RAVER1 (mouse IgG1 isotype) is derived from the hybridoma RAV1 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from mouse immunized with a peptide corresponding to a fragment of human RAVER1. Raver1, also known as ribonucleoprotein PTB-binding 1, is a widely expressed multidomain protein, identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins -actinin and vinculin. The protein is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI.

Application

Monoclonal Anti-RAVER1 antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

RAVER1 (ribonucleoprotein, PTB binding 1) is involved in RNA splicing of microfilament proteins. In skeletal muscle, a translocation of Raver1 from the nucleus to the cytoplasm is correlated with the differentiation of specific microfilament attachment sites. Based on an analysis of Vinculin-Raver1 crystal structure it was suggested that vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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K Meletis et al.
The Journal of cell biology, 155(5), 699-702 (2001-11-29)
Recent studies have shown that cells expressing neuronal antigens can be derived from a bone marrow transplant. A new report lends support to and extends these previous results by presenting compelling morphological evidence for the generation and integration of highly
Raver1 Interactions with Vinculin and RNA Suggest a Feed-Forward Pathway in Directing mRNA to Focal Adhesions
Lee J H, et al.
Structure, 17, 833-842 (2009)
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
Huttelmaier, et al.
The Journal of Cell Biology, 155, 775-775 (2001)

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