SAB4200202
Anti- INI1/SNF5 antibody, Mouse monoclonal
clone 2C2, purified from hybridoma cell culture
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Synonym(s):
Anti-BAF47, Anti-RDT, Anti-RTPS1, Anti-SMARCB1, Anti-SNF5L1, Anti-SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, Anti-Sfh1p, Anti-Snr1, Anti-hSNFS
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
2C2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~44 kDa
species reactivity
monkey, bovine, canine, mouse, rat, human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
western blot: 1.0-2.0 μg/mL using HeLa cell extracts
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SMARCB1(6598)
General description
INI1/SNF5, also known as SWItch/sucrose non-fermentable (SWI/SNF) related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, is encoded by the gene mapped to human chromosome 22q11.2. This gene codes for an essential component of chromatin-remodelling SWI/SNF multiprotein complexes.
Monoclonal Anti-INI1/SNF5 (mouse IgG2a isotype) is derived from the hybridoma 2C2 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from mouse immunized with a human INI1 fusion protein. Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) binds to human immunodeficiency virus type 1 (HIV-1) integrase.
Immunogen
human INI1 fusion protein.
Application
Monoclonal Anti-INI1/SNF5 antibody produced in mouse has been used in immunoblotting and immunocytochemistry.
Biochem/physiol Actions
INI1/sucrose non-fermentable 5 (SNF5) plays a vital role in transcriptional activation of inducible genes through chromatin remodelling. Loss of function mutations of the gene is associated with the pathogenesis of aggressive pediatric cancer and epithelioid schwannoma. The encoded protein triggers DNA-joining activity of the HIV-1 integrase in vitro. Additionally, it might also help in targeting the viral DNA to active genes. SNF5 interacts with E1 protein of human papillomavirus (HPV) and thus, enhances the efficiency of HPV DNA replication.
Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) is recruited to HIV-1 preintegration complexes before nuclear migration and represses the basal human immunodeficiency virus type 1 promoter activity. This gene is a tumor suppressor and mutations in it have been associated with various tumors.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Epigenetics and cancer: altered chromatin remodeling via Snf5 loss leads to aberrant cell cycle regulation
Sansam CG and Roberts CWM
Cell Cycle, 5(6), 621-624 (2006)
Daniel D Shapiro et al.
Clinical and translational medicine, 13(5), e1267-e1267 (2023-05-25)
Renal medullary carcinoma (RMC) is a highly aggressive cancer in need of new therapeutic strategies. The neddylation pathway can protect cells from DNA damage induced by the platinum-based chemotherapy used in RMC. We investigated if neddylation inhibition with pevonedistat will
Integrase interactor 1 (Ini1/hSNF5) is a repressor of basal human immunodeficiency virus type 1 promoter activity
Boese A, et al.
The Journal of General Virology, 90(10), 2503-2512 (2009)
SMARCB1/INI1 Loss in Epithelioid Schwannoma: A Clinicopathologic and Immunohistochemical Study of 65 Cases.
Jo VY and Fletcher CDM
American Journal of Surgical Pathology, 41, 1013-1022 (2017)
Pavlos Msaouel et al.
Cancer cell, 37(5), 720-734 (2020-05-04)
Renal medullary carcinoma (RMC) is a highly lethal malignancy that mainly afflicts young individuals of African descent and is resistant to all targeted agents used to treat other renal cell carcinomas. Comprehensive genomic and transcriptomic profiling of untreated primary RMC
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