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SAB4200201

Sigma-Aldrich

Anti-Cyclophilin B antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-CYP-S1, Anti-CYPB, Anti-OI9, Anti-PPIB, Anti-PPIase B, Anti-S-cyclophilin, Anti-SCYLP, Anti-cyclophilin-like protein, Anti-peptidyl-prolyl cis-trans isomerase B, Anti-peptidylprolyl isomerase B, Anti-rotamase B

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About This Item

MDL number:
MFCD02265740
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~20 kDa

species reactivity

human, rat, monkey, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
western blot: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells

UniProt accession no.

P23284

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PPIB(5479)
mouse ... Ppib(19035)

Related Categories

General description

Anti-Cyclophilin B is produced in rabbit using as the immunogen a synthetic peptide corresponding to a fragment of human Cyclophilin B conjugated to KLH. Cyclophilin B (CypB) is a 21 kDa protein encoded by the PPIB gene, that belongs to the cyclophilin family, a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types. CypB is involved in inflammation, viral infection and cancer.

Application

Anti-Cyclophilin B antibody produced in rabbit has been used:
  • immunoblotting
  • immunofluorescence
  • in immunocapture-polymerase chain reaction (PCR) to study the interactions between CypB and Tomato yellow leaf curl virus (TYLCV) coat protein (CP).

Biochem/physiol Actions

Cyclophilins are peptidyl-prolyl cistrans isomerases (PPIases) that assist protein folding by catalysing the isomerization of peptidyl-proline bonds. Cyclophilin B (CypB) also plays an important role in protecting cells against ER stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Overexpressed cyclophilin B suppresses apoptosis associated with ROS and Ca2+ homeostasis after ER stress
Kim J, et al.
Journal of Cell Science, 121(21), 3636-3648 (2008)
Surapathrudu Kanakala et al.
Frontiers in plant science, 7, 1702-1702 (2016-11-30)
Tomato yellow leaf curl virus (TYLCV) is a single-stranded (ssDNA) begomoviruses that causes severe damage to tomato and several other crops worldwide. TYLCV is exclusively transmitted by the sweetpotato whitefly, Bemisia tabaci in a persistent circulative and propagative manner. Previous
Implication of the whitefly Bemisia tabaci cyclophilin B protein in the transmission of Tomato yellow leaf curl virus
Kanakala S and Ghanim M
Frontiers in Plant Science, 7, 1702-1702 (2016)
Severe osteogenesis imperfecta in cyclophilin B-deficient mice
Choi JW, et al.
PLoS Genetics, 5(12), e1000750-e1000750 (2009)
Xiaoying Ma et al.
Endocrinology and metabolism (Seoul, Korea), 37(3), 432-443 (2022-05-17)
This study investigated the effect of short-chain fatty acids (SCFAs) on diabetes in a mouse model. Autophagy in Akita mice and streptozocin (STZ)-induced diabetic C57BL/6 mice was determined by Western blots and immunohistochemistry (IHC). Western blots, IHC, hematoxylin and eosin
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