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SAB4200189

Sigma-Aldrich

Anti-Sumo-1 antibody, Rat monoclonal

clone 4D12, purified from hybridoma cell culture

Synonym(s):

Anti-DAP1, Anti-GMP1, Anti-OFC10 CLEFT LIP WITH OR WITHOUT CLEFT PALATE, NONSYNDROMIC, 10, Anti-SMT3 suppressor of mif two 3 homolog 1 SENTRIN PIC1 OROFACIAL CLEFT 10, INCLUDED; OFC10, Anti-SMT3H3 SMT3C, Anti-UBL1, SMT3, YEAST, HOMOLOG 3, Anti-small ubiquitin-like modifier 1, Anti-ubiquitin-like 1, Monoclonal Anti-Sumo-1, SUMO-1, SUMO1

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About This Item

MDL number:
MFCD02096449
UNSPSC Code:
12352203
NACRES:
NA.41

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4D12, monoclonal

form

buffered aqueous solution

mol wt

~12 kDa

species reactivity

human, rat, mouse

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
western blot: 2-4 μg/mL using HeLa cell extracts

isotype

IgG2a

UniProt accession no.

P63165

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SUMO1(7341)
mouse ... Sumo1(22216)
rat ... Sumo1(301442)

General description

Monoclonal Anti-Sumo-1 (rat IgG2a isotype) is derived from the hybridoma 4D12 produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from rat immunized with a fusion protein of human SUMO1.
SUMO1 (small ubiquitin-related modifier), also called as Pic1 (inner centromere protein-related protein), Ubl1 (ubiquitin-like protein), hSmt3 (human homolog 3), or sentrin, is mainly expressed in the nucleus. The gene encoding SUMO1 is mapped to human chromosome 2q33. SUMO1 belongs to ubiquitin-like protein family and it consists of 101 amino acids.

Immunogen

fusion protein of human SUMO1

Application

Monoclonal Anti-Sumo-1 has been used in immunocytochemistry.
Monoclonal Anti-Sumo-1 has been used in western blotting.

Biochem/physiol Actions

Sumo-1 interacts with dsDNA and competes with other protein-DNA interactions.
Suppressing SUMO1 (small ubiquitin-related modifier) inhibits multiplication and induces apoptosis of gastric cancer SGC-7901 cells. It is essential for HCV (hepatitis C virus) replication. Deletion of SUMO1 blocks the aggregation of lipid droplets. It is also implicated in mitochondrial fission.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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SUMO1 depletion prevents lipid droplet accumulation and HCV replication.
Akil A
Archives of Virology, 161, 141-148 (2016)
The Transcription Factor EGR1 Localizes to the Nucleolus and Is Linked to Suppression of Ribosomal Precursor Synthesis
Ponti D
PLoS ONE, 9 (2014)
Sumo1 conjugates mitochondrial substrates and participates in mitochondrial fission.
Harder Z
Current Biology, 14, 340-345 (2004)
c-Jun and p53 Activity Is Modulated by SUMO-1 Modification
Muller S
The Journal of Biological Chemistry, 275, 13321-13329 (2000)
The Nucleoporin RanBP2 Has SUMO1 E3 Ligase Activity
Pichler A
Cell, 108, 109-120 (2002)
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