SAB4200186
Anti-DCLK1 antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
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Synonym(s):
Anti-DCAMKL1, Anti-DCDC3A, Anti-DCLK, Anti-doublecortin and CaM kinase-like 1
UNSPSC Code:
12352203
NACRES:
NA.44
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~81 kDa
species reactivity
rat, mouse, human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1.5 mg/mL
technique(s)
western blot: 1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and of HEK-293T cells over-expressing human DCLK1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... DCLK1(9201)
mouse ... Dclk1(13175)
rat ... Dclk1(83825)
Related Categories
General description
Anti-DCLK1 is produced in rabbit using as immunogen a synthetic peptide corresponding to human DCLK1, conjugated to KLH. DCLK1 (doublecortin like kinase 1) is also known as DCLK, DCAMKL1, DCDC3A and CLICK1. DCLK1 consists of an N-terminal doublecortin (DC) domain, responsible for its localization to microtubules and a C-terminal Ser/Thr kinase domain.
DCLK1 is a neuronal transmembrane enzyme that may regulate cortical development, nerve cell migration and microtubule polymerization. This enzyme is similar to DCX, but DCLK1 additionally functions as a Ca2+/calmodulin-dependent protein kinase that can phosphorylate myelin basic protein and itself . Anti-DCLK1 antibody is specific for human, rat and mouse DCLK1. In immunoblotting, detection of the DCLK1 band is specifically inhibited by the DCLK1 immunizing peptide.
Immunogen
The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse DCLK1.
Application
Anti-DCLK1 antibody is suitable for use in western blot (1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and HEK-293T cells over-expressing human DCLK1). The antibody may also be used for immunoblot (approx. 81 kDa) assays.
Biochem/physiol Actions
Doublecortin-like kinase1 (DCLK1) is widely expressed in post-mitotic neurons in the developing nervous system and important for neuronal migration to cerebral cortex. DCLK1 is highly expressed during embryogenesis and is localized to neuronal growth cones where it associates with the microtubule cytoskeleton and regulates microtubule polymerization. DCLK1 kinase domain is susceptible to cleavage by the Ca2+ -dependent protease calpain, suggesting that a Ca2+ -responsive mechanism is involved in regulating the DCLK1 kinase activity during embryogenesis. DCLK1 has been identified as a putative intestinal stem cell marker.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Cleavage of Doublecortin-like Kinase by Calpain Releases an Active Kinase Fragment from a Microtubule Anchorage Domain
Burgess H A and Reiner O
The Journal of biological chemistry, 276(39), 36397-36403 (2001)
Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice
Tuy F P D, wt al.
Developmental Neuroscience, 30(1-3), 171-186 (2008)
DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization
Lin P T, et al.
The Journal of Neuroscience, 20(24), 9152-9161 (2000)
Expression, characterization, and gene knockdown of zebrafish doublecortin-like protein kinase
Shimomura S, et al.
Archives of Biochemistry and Biophysics, 463(2), 218-230 (2007)
Anticancer Activity of Novel Difluorinated Curcumin Analog and Its Inclusion Complex with 2-Hydroxypropyl-I?-Cyclodextrin against Pancreatic Cancer.
Bhattacharyya, et al.
International Journal of Molecular Sciences, 24 (2023)
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