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SAB4200180

Sigma-Aldrich

Anti-Cullin 3 antibody ,Mouse monoclonal

clone CUL3-9, purified from hybridoma cell culture

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Synonym(s):
Anti-CUL3
MDL number:
MFCD03454920
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CUL3-9, monoclonal

form

buffered aqueous solution

mol wt

antigen 80 kDa

species reactivity

rat, human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using whole extracts of human Jurkat cells or mouse brain.

isotype

IgG1

UniProt accession no.

Q13618

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CUL3(8452)
mouse ... Cul3(26554)
rat ... Cul3(301555)

Related Categories

General description

Cullin 3 (CUL3) is a member of cullin protein family and is encoded by the gene mapped to human chromosome 2q36.2. The encoded protein is a core component of cullin/RING E3 ubiquitin ligase (CRL) and is ubiquitously expressed. The protein is characterized with a cullin homology domain.
Monoclonal Anti-Cullin 3 (mouse IgG1 isotype) is derived from the hybridoma CUL3-9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Cullin 3.

Application

Monoclonal Anti-Cullin 3 antibody produced in mouse has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

Cullin 3 (CUL3) acts as a scaffold protein and facilitates the organization of a large number of ubiquitin ligase complexes. It plays a vital role in the regulation of electrolyte homeostasis and blood pressure. The encoded protein acts as a key factor in protein ubiquitination and is also implicated in early embryonic development. One important role of CUL3 is to promote invasion and migration of trophoblast cells. Mutation in the gene leads to onset of pre-eclampsia and pseudohypoaldosteronism type II.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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The CUL3-SPOP-DAXX axis is a novel regulator of VEGFR2 expression in vascular endothelial cells
Sakaue T, et al.
Scientific reports, 7(2), 42845-42845 (2017)
Defining the human sperm microtubulome: an integrated genomics approach
Jumeau F, et al.
Biology of Reproduction, 96(1), 93-106 (2016)
Neddylated Cullin 3 is required for vascular endothelial-cadherin-mediated endothelial barrier function
Sakaue T, et al.
Cancer Science, 108(2), 208-215 (2017)
Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
Boyden LM
Nature, 482, 98-102 (2012)
Tomohisa Sakaue et al.
Scientific reports, 7, 42845-42845 (2017-02-22)
Vascular endothelial cell growth factor receptor 2 (VEGFR2) is an essential receptor for the homeostasis of endothelial cells. In this study, we showed that NEDD8-conjugated Cullin3 (CUL3)-based ubiquitin E3 (UbE3) ligase plays a crucial role in VEGFR2 mRNA expression. Human
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