SAB4200174
Anti-Rab18 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
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All Photos(3)
Synonym(s):
Anti-RAB18LI1, Anti-RP11-148B2.1, Anti-ras-related protein Rab-18
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~23 kDa (additional bands of higher MW may appear in some extract preparations)
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of human PC3 cells
indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells
western blot: 2.5-5.0 μg/mL using whole extracts of mouse MM3MG cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAB18(22931)
mouse ... Rab18(19330)
rat ... Rab18(307039)
General description
Ras-related protein Rab-18 (Rab18) is a member of the ras-associated binding (Rab) family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif, implicated in subcellular targeting. RAB18 gene is mapped to human chromosome 10p12.1.
Specificity
Anti-Rab18 (C-terminal) recognizes human, mouse, and rat Rab18.
Application
Anti-Rab18 (C-terminal) antibody produced in rabbit may be used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Ras-associated binding (Rab)-guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. It localizes to lipid droplets and induces the association of the lipid droplets with endoplasmic reticulum (ER) membranes. Anti-Rab18 may thus be used as a marker to follow the dynamics of lipid droplets in living cells. Rab18 has a potential function in eye development. Mutations in the RAB18 are implicated in Warburg micro syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Discard working dilutions if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Shintaro Ozeki et al.
Journal of cell science, 118(Pt 12), 2601-2611 (2005-05-26)
Lipid droplets (LDs) are organelles that store neutral lipids, but their regulatory mechanism is not well understood. In the present study, we identified Rab18 as an LD component of HepG2 cells by proteomic analysis, and confirmed its localization by immunohistochemistry
Olena Pylypenko et al.
Small GTPases, 9(1-2), 22-48 (2017-06-21)
Rab molecular switches are key players in defining membrane identity and regulating intracellular trafficking events in eukaryotic cells. In spite of their global structural similarity, Rab-family members acquired particular features that allow them to perform specific cellular functions. The overall
A case report of a patient with interstitial duplication of 10p12. 1 and 15q11. 2q13. 1
Park HY, et al.
European Journal of Paediatric Neurology, 21, e48-e48 (2017)
Selma Yilmaz Dejgaard et al.
Cellular and molecular life sciences : CMLS, 76(10), 1935-1945 (2019-03-05)
Rab18 is one of the small number of conserved Rab proteins which have been traced to the last eukaryotic common ancestor. It is found in organisms ranging from humans to trypanosomes, and localizes to multiple organelles, including most notably endoplasmic
Danai Bem et al.
American journal of human genetics, 88(4), 499-507 (2011-04-09)
Warburg Micro syndrome and Martsolf syndrome are heterogenous autosomal-recessive developmental disorders characterized by brain, eye, and endocrine abnormalities. Previously, identification of mutations in RAB3GAP1 and RAB3GAP2 in both these syndromes implicated dysregulation of the RAB3 cycle (which controls calcium-mediated exocytosis
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