SAB4200151
Anti-Drosha antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
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All Photos(2)
Synonym(s):
Anti-ETOHI2, RANSE3L, Anti-RN3, Anti-RNASEN, Anti-RNC, Anti-RNase III, Anti-p241, Anti-ribonuclease type III, nuclear
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 130-160 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells.
western blot: 2-4 μg/mL using HeLa nuclear cell lysates.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RNASEN(29102)
General description
Drosha ribonuclease III (Drosha) is encoded by the gene mapped to human chromosome 5p13.3. It is a double-stranded RNA (dsRNA)-specific RNase III enzyme. The encoded protein contains RNase III domains (RIIIDs) on the top and central domain (CED) at the bottom. In addition, it also contains unique non-canonical zinc-finger motifs.
Application
Anti-Drosha antibody produced in rabbit has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA).Drosha ribonuclease III (Drosha) plays a vital role in initiation of microRNA (miRNA) maturation. It catalyzes the cleavage of stem loop structure from the primary microRNA (pri-miRNA). Mutation in the gene is associated with development of Epithelial ovarian cancer (EOC). Knockdown of Drosha can enhance the sensitivity of gastric cancer to epirubicin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Genome-wide Mapping of DROSHA Cleavage Sites on Primary MicroRNAs and Noncanonical Substrates
Kim B
Molecular Cell, 66, 258-269 (2017)
[Knockdown of Drosha promotes chemosensitivity of epirubicin for gastric cancer MGC-803 cells].
Xu L
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 32, 1207-1211 (2016)
A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
Fletcher CE
Nucleic Acids Research (2016)
Peike Sheng et al.
Nucleic acids research, 46(11), 5737-5752 (2018-05-11)
MicroRNAs (miRNAs) are approximately 22 nucleotide (nt) long and play important roles in post-transcriptional regulation in both plants and animals. In animals, precursor (pre-) miRNAs are ∼70 nt hairpins produced by Drosha cleavage of long primary (pri-) miRNAs in the
Dongli Pan et al.
mBio, 10(1) (2019-02-14)
Herpes simplex virus 1 (HSV-1) switches between two infection programs, productive ("lytic") and latent infection. Some HSV-1 microRNAs (miRNAs) have been hypothesized to help control this switch, and yet little is known about regulation of their expression. Using Northern blot
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