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SAB4200135

Sigma-Aldrich

Anti-FIP200 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody

Synonym(s):

Anti-200 kDa FAK family kinase-interacting protein, Anti-CC1, Anti-DRAGOU14, Anti-RB1-inducible coiled-coil 1, Anti-RB1CC1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

canine, mouse, bovine, monkey, rat, human

concentration

~1 mg/mL

technique(s)

western blot: 1-2 μg/mL using whole extracts of HEK-293 cells overexpressing human FIP200

UniProt accession no.

Q8TDY2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RB1CC1(9821)
mouse ... Rb1cc1(12421)
rat ... Rb1cc1(312927)

General description

FIP200 (focal adhesion kinase family interacting protein of 200 kDa), also known as RB1CC1 (retinoblastoma 1-inducible coiled-coil 1), is a multifunctional protein. It is mainly localized in the cytoplasm. The FIP200 gene is mapped to human chromosome 8q11.23.

Specificity

Anti-FIP200 (C-terminal) recognizes human FIP200.

Application

Anti-FIP200 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) interacts with UNC-51-like kinase (ULK 1) and is essential for autophagy. It also interacts with Pyk2 (proline-rich tyrosine kinase 2), FAK (focal adhesion kinase), TSC1 (TSC complex subunit 1), p53, apoptosis signal-regulating kinase 1 (ASK1) and TRAF2 (TNF receptor associated factor 2) and regulates cell growth, cell proliferation, cell survival, cell adhesion and cell migration. Deficiency in FIP200 is associated with embryonic death and cancer development.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
077M4795V
077M4765V
040M4766

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Christopher P Webster et al.
The EMBO journal, 35(15), 1656-1676 (2016-06-24)
A GGGGCC hexanucleotide repeat expansion in the C9orf72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia (C9ALS/FTD). C9orf72 encodes two C9orf72 protein isoforms of unclear function. Reduced levels of C9orf72 expression have been reported
FIP200, a key signaling node to coordinately regulate various cellular processes
Gan B and Guan JL
Cellular Signalling, 20(5), 787-794 (2008)
The role of the Atg1/ULK1 complex in autophagy regulation
Mizushima N
Current Opinion in Cell Biology, 22(2), 132-139 (2010)
Mahipal V Suraneni et al.
Cell cycle (Georgetown, Tex.), 13(11), 1798-1810 (2014-04-16)
15-Lipoxygenase-2 (15-LOX2) is a human-specific lipid-peroxidizing enzyme most prominently expressed in epithelial cells of normal human prostate but downregulated or completely lost in>70% of prostate cancer (PCa) cases. Transgenic expression of 15-LOX2 in the mouse prostate surprisingly causes hyperplasia. Here
Xin Yu et al.
Journal of molecular neuroscience : MN, 50(3), 586-599 (2013-04-18)
Spinocerebellar ataxia type 7 (SCA7) is one of nine neurodegenerative disorders caused by expanded polyglutamine domains. These so-called polyglutamine (polyQ) diseases are all characterized by aggregation. Reducing the level of aggregating polyQ proteins via pharmacological activation of autophagy has been
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