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SAB4200124

Sigma-Aldrich

Anti-Nexilin antibody,Mouse monoclonal

clone NX-38, purified from hybridoma cell culture

Synonym(s):

Anti-NEXN, Anti-Nelin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NX-38, monoclonal

form

buffered aqueous solution

mol wt

antigen 80 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable
western blot: 1-2 μg/mL using using rat brain S1 fraction extracts

isotype

IgG2a

UniProt accession no.

Q0ZGT2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEXN(91624)
mouse ... Nexn(68810)
rat ... Nexn(246172)

General description

Monoclonal Anti-Nexilin (mouse IgG2a isotype) is derived from the hybridoma NX-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide conjugated to KLH. Nexilin is a filamentous actin-binding protein that localizes to focal contacts. Alternative splicing of the gene yields two different isoforms namely, b-nexilin and s-nexilin.
NEXN (nexilin), a cardiac Z-disc protein is made of 675 amino acids. It is present in the heart and skeletal muscle. NEXN is mapped to human chromosome 1p31.

Specificity

Monoclonal Anti-Nexilin recognizes human, rat, and mouse nexilin.

Application

Anti-Nexilin antibody,Mouse monoclonal may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

NEXN (nexilin) plays an important role in protecting cardiac Z-discs from forces generated within the sarcomere. Mutations in NEXN results in hypertrophic cardiomyopathy.
Nexilin may be involved in cell adhesion and migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Mutations in NEXN, a Z-disc gene, are associated with hypertrophic cardiomyopathy.
Wang H, et al.
American Journal of Human Genetics, 87(5), 687-693 (2010)
A novel genetic variant in long non-coding RNA gene NEXN-AS1 is associated with risk of lung cancer.
Yuan H, et al.
Scientific Reports, 6, 34234-34234 (2016)
H T Law et al.
Cellular microbiology, 14(7), 1097-1108 (2012-03-03)
The bacterial pathogens Listeria monocytogenes and enteropathogenic Escherichia coli (EPEC) generate motile actin-rich structures (comet tails and pedestals) as part of their infectious processes. Nexilin, an actin-associated protein and a component of focal adhesions, has been suggested to be involved

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