SAB4200100
Anti-ATG13 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
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Synonym(s):
ATG13 Antibody - Anti-ATG13 antibody produced in rabbit, Atg13 Antibody, Anti-Autophagy-related protein 13
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human, rat, mouse
concentration
~1.0 mg/mL
technique(s)
western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KIAA0652(9776)
Related Categories
General description
Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).
Specificity
Anti-Atg13 recognizes human, mouse, and rat Atg13.
Application
Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.
Biochem/physiol Actions
Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Wei Lu et al.
Stem cells and development, 24(9), 1133-1140 (2014-12-02)
The 3' untranslated region (UTR)-associated RNAs (uaRNAs) have important roles in various biological processes, especially in development. However, since they overlap with protein-coding mRNAs, uaRNAs are difficult to study by RNA interference techniques. We recently identified a chemical molecule, 3-benzyl-5-((2-nitrophenoxy)
Physical and neurobehavioral determinants of reproductive onset and success
Day FR, et al.
Nature Genetics, 48(6), 617-617 (2016)
Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform
Zachari M, et al.
Developmental Cell (2019)
Systematic analysis of ATG13 domain requirements for autophagy induction
Wallot-Hieke N, et al.
Autophagy, 14(5), 743?763-743?763 (2018)
Di Ge et al.
Autophagy, 10(6), 957-971 (2014-06-01)
MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge
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