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SAB4200093

Sigma-Aldrich

Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal

clone 8B3-C3-E4, purified from hybridoma cell culture

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Synonym(s):
Anti-Dedicator of cytokinesis 9, Anti-ZIZ1
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

8B3-C3-E4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~236 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 3.0-6.0 μg/mL using MDA-MB-231 cell extracts

UniProt accession no.

Q9BZ29

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DOCK9(23348)

General description

Monoclonal Anti-DOCK9 (Zizimin1) (mouse IgG2a isotype) is derived from the hybridoma 8B3-C3-E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Zizimin1 is a guanine nucleotide exchange factor (GEF) specific to Cdc42. It is a part of the second family of mammalian Rho-GEFs, CZH [CDM (Ced-5/DOCK180/Myoblast city)-zizimin homology] proteins. The Zizimin1 protein contains the CZH2 domain, CZH1 domain and Pleckstrin homology (PH) domain.

Specificity

Monoclonal Anti-DOCK9 (Zizimin1) recognizes human DOCK9.

Immunogen

full length DOCK9 (Zizimin1)

Application

Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal is suitable for:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

Zizimin1/ dedicator of cytokinesis 9 (DOCK9) is found to activate Cdc42 through binding to its CZH2 domain. Interestingly, the CZH1 domain of Zizimin1 at the N-terminal binds to its CZH2 domain and thus inhibits Cdc42 binding. The Pleckstrin homology (PH) domain at the N-terminal, binds phosphoinositides and results in zizimin1 membrane targeting.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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The novel Cdc42 guanine nucleotide exchange factor, zizimin1, dimerizes via the Cdc42-binding CZH2 domain
Meller N, et al.
Test, 279(36), 37470-37476 (2004)
Function of the N-terminus of zizimin1: autoinhibition and membrane targeting
Meller N, et al.
The Biochemical Journal, 409(2), 525-533 (2008)

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