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Merck
CN

SAB4200087

Anti-Dicer antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Dicer1, Anti-Endoribonuclease Dicer, Anti-HERNA, Anti-Helicase with RNase motif

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Dicer antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~218 kDa

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 3-6 μg/mL using HeLa cell lysates

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DICER1(23405)
mouse ... Dicer1(192119)

Application

Anti-Dicer antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescent staining

Biochem/physiol Actions

Anti-Dicer recognizes human Dicer.
Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.
Mutation in the Dicer gene causes malignancies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.
Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000282623
0000282623
57091109
0000138648
117M4839V

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Dicer cleaves 5?-extended microRNA precursors originating from RNA polymerase II transcription start sites
Sheng P, et al.
Nucleic Acids Research, 46(11), 5737-5752 (2018)
The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development
Antoniou A, et al.
EMBO Reports, 19(3) (2018)
Simion Chiosea et al.
The American journal of pathology, 169(5), 1812-1820 (2006-10-31)
MicroRNAs are small noncoding 18- to 24-nt RNAs that are predicted to regulate expression of as many as 30% of protein-encoding genes. In prostate adenocarcinoma, 39 microRNAs are up-regulated, and six microRNAs are down-regulated. Production and function of microRNA requires
Molecular mechanisms of Dicer: endonuclease and enzymatic activity
Song MS and Rossi JJ
The Biochemical Journal, 474(10), 1603-1618 (2017)
DICER regulates the expression of major satellite repeat transcripts and meiotic chromoso
Yadav RP, et al.
Nucleic Acids Research, 48(13), 7135-7153 (2020)
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