Skip to Content
Merck
CN
All Photos(4)

Documents

Safety Information

SAB4200087

Sigma-Aldrich

Anti-Dicer antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
Anti-Dicer1, Anti-Endoribonuclease Dicer, Anti-HERNA, Anti-Helicase with RNase motif
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~218 kDa

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 3-6 μg/mL using HeLa cell lysates

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DICER1(23405)
mouse ... Dicer1(192119)

Related Categories

General description

Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.
Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.

Specificity

Anti-Dicer recognizes human Dicer.

Application

Anti-Dicer antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescent staining

Biochem/physiol Actions

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.
Mutation in the Dicer gene causes malignancies.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development
Antoniou A, et al.
EMBO Reports, 19(3) (2018)
Dicer cleaves 5?-extended microRNA precursors originating from RNA polymerase II transcription start sites
Sheng P, et al.
Nucleic Acids Research, 46(11), 5737-5752 (2018)
Evolutionarily conserved roles of the dicer helicase domain in regulating RNA interference processing
Kidwell MA, et al.
The Journal of Biological Chemistry, 289(41), 28352-28362 (2014)
RNAi pathway participates in chromosome segregation in mammalian cells
Huang C, et al.
Cell discovery, 1, 15029-15029 (2015)
Molecular mechanisms of Dicer: endonuclease and enzymatic activity
Song MS and Rossi JJ
The Biochemical Journal, 474(10), 1603-1618 (2017)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service