SAB4200084
Monoclonal Anti-AGO1 antibody produced in rat
~1 mg/mL, clone 4B8, purified immunoglobulin
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Synonym(s):
Anti-Argonaute-1, Anti-Eukaryotic Translation Initiation Factor 2c, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4B8, monoclonal
form
buffered aqueous solution
mol wt
antigen ~95 kDa
species reactivity
human, canine, mouse, monkey, bovine
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using HEK-293T cell extracts
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EIF2C1(26523)
mouse ... Eif2c1(236511)
General description
Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1−4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.
Immunogen
recombinant human AGO1 fusion protein.
Application
- Monoclonal Anti-AGO1 antibody produced in rat has been used in:
- western blotting
- immunoprecipitation
- chromatin immunoprecipitation(CHIP) assay
- RNA immunoprecipitation
- RNA-nChIP experiments
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
Biochem/physiol Actions
The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Mariano Alló et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(44), 15622-15629 (2014-10-15)
The roles of Argonaute proteins in cytoplasmic microRNA and RNAi pathways are well established. However, their implication in small RNA-mediated transcriptional gene silencing in the mammalian cell nucleus is less understood. We have recently shown that intronic siRNAs cause chromatin
Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis
Chen Z, et al.
The Journal of Clinical Investigation, 123(3), 1057-1057 (2013)
Argonaute protein as a linker to command center of physiological processes
Wei K, et al.
Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu, 25(4), 430-430 (2013)
Michaela Beitzinger et al.
RNA biology, 4(2), 76-84 (2007-07-20)
MicroRNAs (miRNAs) constitute a class of small non-coding RNAs that regulate gene expression on the level of translation and/or mRNA stability. Mammalian miRNAs associate with members of the Argonaute (Ago) protein family and bind to partially complementary sequences in the
The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
Kratassiouk G, et al.
Cell Cycle, 15(5), 667-677 (2016)
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