Anti-Vinculin antibody, Mouse monoclonal

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma V284 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified human platelet vinculin. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), neuromuscular junctions, myotendinous junctions (skeletal muscle) and the membrane-associated dense bodies of smooth muscle.

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

purified human platelet vinculin.

Anti-Vinculin antibody, Mouse monoclonal has been used:

• to probe blots in immunoblotting
• immunochemical staining
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting.

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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