Skip to Content
Merck
CN
All Photos(3)

Key Documents

View All Documentation

Safety Information

SAB4200079

Sigma-Aldrich

Anti-IQGAP1 (C-terminal region) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-HUMORFA01, Anti-IQ motif-containing GTPase-activating protein 1, Anti-RASGAP-like with IQ motifs, Anti-SAR1, Anti-p195

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~189 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunohistochemistry: 20-40 μg/mL using formalin-fixed and paraffin embedded human colon
immunoprecipitation (IP): 10-20 μg using HeLa cell lysates
western blot: 2-4 μg/mL using using MCF7 cell extracts

UniProt accession no.

P46940

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IQGAP1(8826)

General description

IQ motif containing GTPase activating protein 1(IQGAP1) is encoded by the gene mapped to human chromosome 15q26.1. The encoded 190kDa protein is widely expressed and is mainly localized to lamellipodia and membrane ruffles in fibroblasts and other cell types.
IQGAP1 is a scaffolding protein and is made up of N-terminal calponin-homology domain (CHD), WW domain and guanosine triphosphatase (GTPase) activating protein GAP-related domain (GRD).

Specificity

Anti-IQGAP1 (C-terminal region) specifically recognizes human IQGAP1.

Application

Anti-IQGAP1 (C-terminal region) antibody produced in rabbit may be used in:
  • immunoprecipitation
  • western blotting
  • immunohistochemistry

Biochem/physiol Actions

IQ motif containing GTPase activating protein 1(IQGAP1) interacts with cell division control protein 42 homolog (Cdc42) or Rac1 and regulate cadherin-based cell adhesion. IQGAP1 binds to NLRC3 in human monocytic and epithelial cells and regulate type I interferon (IFN) production. Overexpression of the gene has been observed in gastric cancer cell lines.In addition, IQGAP1 is also associated with diseases, such as cancer and diabetes. The encoded protein is also implicated in the production and secretion of insulin. IQGAP1 also aids in the endocytosis and plasma membrane fusion of vesicles in pancreatic β-cells.
IQGAP1 plays a role in tumorigenesis. IQGAP1 gene is upregulated in several carcinomas, including colorectal, lung, ovary, and breast carcinoma.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
020M4767

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nucleotide variants within the IQGAP1 gene in diffuse-type gastric cancers.
Morris L E
Genes Chromosomes Cancer, 42, 280-286 (2005)
Additional complexity on human chromosome 15q: identification of a set of newly recognized duplicons (LCR15) on 15q11-q13, 15q24, and 15q26.
Pujana MA
Genome Research, 11, 98-111 (2001)
Lorraine Jadeski et al.
The Journal of biological chemistry, 283(2), 1008-1017 (2007-11-06)
The scaffold protein IQGAP1 integrates signaling pathways and participates in diverse cellular activities. IQGAP1 is overexpressed in a number of human solid neoplasms, but its functional role in tumorigenesis has not been previously evaluated. Here we report that IQGAP1 contributes
Peixin Dong et al.
Cancer letters, 243(1), 120-127 (2006-01-03)
IQGAP1 is a multifunctional protein involved in actin cytoskeleton assembly and E-cadherin-mediated cell adhesion. To determine the role of IQGAP1 in ovarian tumors, we evaluated IQGAP1 expression by immunohistochemistry in 17 adenomas, 30 borderline tumors and 80 adenocarcinomas and its
Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1.
Li S
Molecular and Cellular Biology, 20, 697-701 (2000)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service