SAB4200079
Anti-IQGAP1 (C-terminal region) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody
Synonym(s):
Anti-HUMORFA01, Anti-IQ motif-containing GTPase-activating protein 1, Anti-RASGAP-like with IQ motifs, Anti-SAR1, Anti-p195
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About This Item
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, IP, WB
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~189 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1.5 mg/mL
technique(s)
immunohistochemistry: 20-40 μg/mL using formalin-fixed and paraffin embedded human colon, immunoprecipitation (IP): 10-20 μg using HeLa cell lysates, western blot: 2-4 μg/mL using using MCF7 cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IQGAP1(8826)
General description
IQ motif containing GTPase activating protein 1(IQGAP1) is encoded by the gene mapped to human chromosome 15q26.1. The encoded 190kDa protein is widely expressed and is mainly localized to lamellipodia and membrane ruffles in fibroblasts and other cell types.
IQGAP1 is a scaffolding protein and is made up of N-terminal calponin-homology domain (CHD), WW domain and guanosine triphosphatase (GTPase) activating protein GAP-related domain (GRD).
Application
Anti-IQGAP1 (C-terminal region) antibody produced in rabbit may be used in:
- immunoprecipitation
- western blotting
- immunohistochemistry
Biochem/physiol Actions
Anti-IQGAP1 (C-terminal region) specifically recognizes human IQGAP1.
IQ motif containing GTPase activating protein 1(IQGAP1) interacts with cell division control protein 42 homolog (Cdc42) or Rac1 and regulate cadherin-based cell adhesion. IQGAP1 binds to NLRC3 in human monocytic and epithelial cells and regulate type I interferon (IFN) production. Overexpression of the gene has been observed in gastric cancer cell lines.In addition, IQGAP1 is also associated with diseases, such as cancer and diabetes. The encoded protein is also implicated in the production and secretion of insulin. IQGAP1 also aids in the endocytosis and plasma membrane fusion of vesicles in pancreatic β-cells.
IQGAP1 plays a role in tumorigenesis. IQGAP1 gene is upregulated in several carcinomas, including colorectal, lung, ovary, and breast carcinoma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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The Scaffolding Protein IQGAP1 Interacts with NLRC3 and Inhibits Type I IFN Production.
Tocker AM, et.al.
Journal of Immunology, 199, 2896-2909 (2017)
Scaffolding protein IQGAP1: an insulin-dependent link between caveolae and the cytoskeleton in primary human adipocytes?
Jufvas ?
The Biochemical Journal, 473, 3177-3188 (2016)
Lorraine Jadeski et al.
The Journal of biological chemistry, 283(2), 1008-1017 (2007-11-06)
The scaffold protein IQGAP1 integrates signaling pathways and participates in diverse cellular activities. IQGAP1 is overexpressed in a number of human solid neoplasms, but its functional role in tumorigenesis has not been previously evaluated. Here we report that IQGAP1 contributes
Peixin Dong et al.
Cancer letters, 243(1), 120-127 (2006-01-03)
IQGAP1 is a multifunctional protein involved in actin cytoskeleton assembly and E-cadherin-mediated cell adhesion. To determine the role of IQGAP1 in ovarian tumors, we evaluated IQGAP1 expression by immunohistochemistry in 17 adenomas, 30 borderline tumors and 80 adenocarcinomas and its
M J Hart et al.
The EMBO journal, 15(12), 2997-3005 (1996-06-17)
Proteins that associate with the GTP-bound forms of the Ras superfamily of proteins are potential effector targets for these molecular switches. A 195 kDa protein was purified from cell lysates by affinity chromatography on immobilized cdc42Hs-GTP and a corresponding cDNA
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