SAB4200041
Anti-Talin antibody,Mouse monoclonal
clone TA205, purified from hybridoma cell culture
Synonym(s):
Anti-TLN
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TA205, monoclonal
form
buffered aqueous solution
mol wt
antigen ~225 kDa
species reactivity
chicken, bovine, human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 0.5-1 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa/Jurkat cell extracts
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
chicken ... Tln1(395194)
human ... TLN1(7094)
General description
Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the hybridoma TA205 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with partially purified preparation of human platelet talin. Talin is present in areas of cell-substratum and cell-cell contacts. Two types of talin were found in human, talin 1 and talin 2. Talin 1 co-distributes with integrins in the cell surface membrane. The C-terminus comprises binding sites for proteins such as β-1-integrin, actin, and vinculin. The N-terminus of the protein consists of elements for localization to cell extracellular matrix junctions. Talin 2 has a different pattern of expression compared to talin 1, but talin1 associates with unique transmembrane receptors to form novel linkages between extracellular matrices and actin cytoskeleton.
Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.
Immunogen
partially purified preparation of human platelet talin
Application
Monoclonal Anti-Talin antibody produced in mouse has been used in western blotting and immunostaining.
Monoclonal Anti-Talin antibody produced in mouse has been used in:
- Immunoprecipitation
- immunofluorescence
- immunocytochemistry
Biochem/physiol Actions
Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent) -resistance.
Talin 1 assists in the attachment of adherent cells to the extracellular matrix and of lymphocytes to other cells. It plays an important role in the assembly of actin filaments and in spreading and migration of different cell types, such as fibroblasts and osteoclasts.
Target description
Talin is a cytoskeletal protein that is found concentratedin areas of cell-substratum and cell-cell contacts. Theencoded protein plays a significant role in the assemblyof actin filaments and in spreading and migration ofvarious cell types, includ
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
Gene duplication and functional divergence during evolution of the cytoskeletal linker protein talin
Senetar MA and McCann RO
Gene, 362, 141-152 (2005)
Ming-Tsung Lai et al.
The Journal of pathology, 224(3), 367-376 (2011-05-07)
Oral squamous cell carcinoma (OSCC) is highly invasive and is associated with frequent tumour recurrences and lymph node metastases. Identification of genes involved in the aggressiveness of OSCC may provide new targets for clinical intervention. A genome-wide study based on
Ayman Alsebaey et al.
Asian Pacific journal of cancer prevention : APJCP, 17(8), 4077-4082 (2016-09-21)
Hepatocellular carcinoma (HCC) is a dreadful complication of end stage liver disease with high morbidity and mortality. The aim was to assess the role of serum talin-1 and non-invasive brosis in patients with HCC. A total of eighty seven subjects
YAP regulates cell mechanics by controlling focal adhesion assembly
Nardone G
Nature Communications (2017)
High expression of Talin-1 is associated with poor prognosis in patients with nasopharyngeal carcinoma.
Xu YF
BMC Cancer (2015)
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