SAB4200025
Anti-VPS4 antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
All Photos(6)
Synonym(s):
Anti-Vacuolar protein sorting 4
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
rat, human, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2-4 μg using lysates of human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or rat NRK cells
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VPS4A(27183)
mouse ... Vps4a(116733)
rat ... Vps4a(246772)
General description
Vacuolar protein sorting-associated protein 4 (Vps4) is a member of the AAA protein family (ATPases associated with diverse cellular activities). Vps4 exists as two orthologs in humans (VPS4A and VPS4B). The gene encoding Vps4A is mapped to chromosome 16q22, while the gene for Vps4B resides on chromosome 18q21-q22.
Specificity
Anti-VPS4 recognizes human, mouse, and rat VPS4A and VPS4B.
Application
Anti-VPS4 antibody has been used in
- immunoprecipitation
- immunofluorescence Confocal Microscopy
- immunohistochemistry
- western blotting
Biochem/physiol Actions
Vacuolar protein sorting-associated protein 4 (VPS4) mediates endosomal membrane protein sorting, retroviral budding and cytokinesis by recognizing membrane-associated ESCRT-III assemblies and catalyzing their disassembly.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Shimon Harel et al.
Biophysical journal, 121(21), 4229-4238 (2022-09-01)
The assembly and budding of newly formed human immunodeficiency virus-1 (HIV-1) particles occur at the plasma membrane of infected cells. Although the molecular basis for viral budding has been studied extensively, investigation of its spatiotemporal characteristics has been limited by
ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4
Thoresen SB, et al.
Nature Cell Biology, 16(6), 550-560 (2014)
The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting of alpha-synuclein
Hasegawa T, et al.
PLoS ONE, 6(12), e29460-e29460 (2011)
Aiqin Hu et al.
BMC genetics, 20(1), 7-7 (2019-01-13)
Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the ATP enzyme AAA protein family, and is mainly involved in protein degradation and cell membrane fusion. Recently, a dominant mutation in this gene was identified in human dentin dysplasia
S Scheuring et al.
Journal of molecular biology, 312(3), 469-480 (2001-09-21)
The yeast Vps4 protein (Vps4p) is a member of the AAA protein family (ATPases associated with diverse cellular activities) and a key player in the transport of proteins out of a prevacuolar endosomal compartment. In human cells, we identified two
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service