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SAB4200022

Sigma-Aldrich

Anti-VPS4A antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SKD1, Anti-SKD1-homolog, Anti-SKD1A, Anti-SKD2, Anti-VPS4, Anti-VPS4-1, Anti-Vacuolar protein sorting 4 homolog A

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 10-20 μg using lysates of rat NRK cells
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over expressing human VPS4A

UniProt accession no.

Q9UN37

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VPS4A(27183)
mouse ... Vps4a(116733)
rat ... Vps4a(246772)

General description

Vacuolar-protein-sorting factor 4A (VPS4A) is an AAAtype ATPase, encoded by the gene mapped to human chromosome 16q22.1. The encoded protein is a member of vacuolar sorting protein family. The protein is characterized by a canonical AAA ATPase cassette followed by an N-terminal MIT (microtubule interacting and trafficking) domain.

Application

Anti-VPS4A antibody produced in rabbit has been used for co-immunoprecipitation and Western blotting.

Biochem/physiol Actions

Vacuolar-protein-sorting factor 4A (VPS4A) plays a vital role in lysosomal/endosomal membrane trafficking. It is also involved in the regulation of exosome biogenesis. Downregulated expression of the gene leads to the development of hepatocellular carcinoma (HCC). Thus, VPS4 is considered to be an effective tumor suppressor for HCC. Mutation of the gene leads to defective multivesicular body formation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

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Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A
Anna Scott
Proceedings of the National Academy of Sciences of the USA (2005)
A fourth locus for autosomal dominant hypercholesterolemia maps at 16q22.1
Alice M
European Journal of Human Genetics (2010)
Disruption of Neurexin 1 Associated with Autism Spectrum Disorder
Hyung-GooKim
Academic Journal of Interdisciplinary Studies (2008)
Exosomes mediate cell contact?independent ephrin-Eph signaling during axon guidance
Jingyi Gong
The Journal of Cell Biology (2016)
Jingyi Gong et al.
The Journal of cell biology, 214(1), 35-44 (2016-06-30)
The cellular release of membranous vesicles known as extracellular vesicles (EVs) or exosomes represents a novel mode of intercellular communication. Eph receptor tyrosine kinases and their membrane-tethered ephrin ligands have very important roles in such biologically diverse processes as neuronal
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