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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Technique(s):
western blot: 1:1000
Application:
WB
Citations:
10
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
western blot: 1:1000
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
Histidine has a strong association with immobilized metal ion matrices. This is because the imidazole ring has electron donor groups that form coordination bonds with the transition metal that is immobilized. Hexahistidine tag (His-tag) consists of four to six histidines that are fused genetically. It is mainly used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). His-tags are added at the amino or carboxy termini of proteins.
Physical form
Supplied in PBS with 0.09% (W/V) sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
常规特殊物品
低风险生物材料
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Yuria Jang et al.
International journal of molecular medicine, 53(1) (2023-12-08)
The binding between receptor‑activated nuclear factor‑κB (RANK) and the RANK ligand (RANKL) during osteoclast development is an important target for drugs that treat osteoporosis. The leucine‑rich repeat‑containing G‑protein‑coupled receptor 4 (LGR4) acts as a negative regulator of RANK‑RANKL that suppresses
Shuting Ding et al.
The EMBO journal, 42(6), e111858-e111858 (2022-12-24)
Phytosulfokine (PSK) is a plant pentapeptide hormone that fulfills a wide range of functions. Although PSK has frequently been reported to function in the inverse regulation of growth and defense in response to (hemi)biotrophic pathogens, the mechanisms involved remain largely
[16] Purification of Proteins Using Polyhistidine Affinity Tags
Joshua A, et al.
Methods in Enzymology (2000)
Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins? Evaluation of Two Anti-His-Tag Monoclonal Antibodies
Kristian M.Muller, et al.
Analytical Biochemistry, 54-61 (1998)
Sebastian Dorawa et al.
International journal of molecular sciences, 23(14) (2022-07-28)
We present a structural and functional analysis of the DNA polymerase of thermophilic Thermus thermophilus MAT72 phage vB_Tt72. The enzyme shows low sequence identity (<30%) to the members of the type-A family of DNA polymerases, except for two yet uncharacterized
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