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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Product Name
Sulfatase from Helix pomatia, Type H-2, aqueous solution, ≥2,000 units/mL
type
Type H-2
form
aqueous solution
secondary activity
≥100,000 units/mL β-glucuronidase (at pH 5.0)
mol wt
~85 kDa by gel filtration
concentration
≥2,000 units/mL
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Application
Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.
General description
Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Fernando Cámara-Martos et al.
Foods (Basel, Switzerland), 11(3) (2022-02-16)
In northwest Spain and Portugal, there is a long tradition of cultivating B. rapa subsp. rapa to obtain turnip greens and turnip tops. Brassica rapa L. subsp. rapa (turnip greens and turnip tops) were grown under conventional and organic conditions
Jakub Kopycki et al.
Biotechnology letters, 33(5), 1039-1046 (2011-01-27)
Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action
Abigail M Tokheim et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 18(5), 537-540 (2005-12-08)
Often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from Helix pomatia remain little characterized. As many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the
Tania Merinas-Amo et al.
Foods (Basel, Switzerland), 10(11) (2021-11-28)
Brassica rapa L. subsp. rapa (turnip greens), a traditionally consumed vegetable, is well-known due to its high content of glucosinolates, which are secondary metabolites with a positive biological activity for human health. Our hypothesis has been based on the relation
G Pelsy et al.
Biotechnology and bioengineering, 25(4), 919-928 (1983-04-01)
It has been found that sulfatase from Helix pomatia hydrolyzes beta-naphthyl sulfate much faster than alpha-naphthyl sulfate; e.g., at pH 7.8, while the former is readily hydrolyzed, the latter undergoes no appreciable hydrolysis. Kinetic investigations of both enzymatic and acid
Protocols
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Related Content
Instructions
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