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S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type H-1

Quality Level

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction
  • sulfatase assays
  • enzymatic hydrolysis
  • extract preparation for glucosinolate analysis

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Product S9626, Sulfatase from Helix pomatia?

    Prior to determining the activity of this enzyme, Sigma reconstitutes it in 0.2% (w/v) sodium chloride.

  6. Does Product S9626, Sufatase from Helix pomatia, contain B-Glucuronidase activity?

    This product contains >300 units/mg solid B-Glucuronidase activity.

  7. How is Product S9626, Sulfatase from helix pomatia, prepared?

    This enzyme is isolated by acetone fractionation.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction
Hirai T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 692(2), 375-388 (1997)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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