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S8820

Sigma-Aldrich

SIGMAFAST™ Protease Inhibitor Tablets

For General Use

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Synonym(s):
Protease Inhibitor Tablets, protease inhibitor
UNSPSC Code:
12352200
NACRES:
NA.77

biological source

bovine lung
bovine milk

Quality Level

200

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

Related Categories

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:
  • to wash raw semen for membrane protein analysis
  • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
  • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
  • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Features and Benefits

  • Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
  • Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
  • Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
  • Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

P8849

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8830

SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference between Protease Inhibitors S8820 and P2714?

    S8820 is the tablet version of P2714.  

  6. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?

    The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).

  7. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with ELISA?

    Yes, because it is water based.

  8. How should I dissolve Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.

  9. What is the concentration of the inhibitors in Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF      2 mME-64          14 μMBestatin     130 μMLeupeptin   0.9 μMAprotinin    0.3 μMEDTA        1 mM

  10. How much of Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, needs to be used in cell lysates?

    One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The spinal cord injury (SCI) is a detrimental neurological disease involving the primary mechanical injury and secondary inflammatory damage. Curtailing the detrimental neuroinflammation would be beneficial for spinal cord function recovery. Microglia reside in the spinal cord and actively participate
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Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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