S8447
Anti-Sirt2 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-SIR2L, Anti-SIR2L2, Anti-Sirtuin (silent mating type information regulation 2 homolog), Anti-Sirtuin 2
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 37:43 kDa (rat)
antigen 43 kDa (human)
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): 2-5 μg using extract of COS7 cells or 293 cells expressing recombinant human SIRT2 protein.
indirect immunofluorescence: 5-10 μg/mL using human HeLa cells.
western blot: 1-2 μg/mL using whole extracts of mouse and rat brain.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SIRT2(22933)
mouse ... Sirt2(64383)
rat ... Sirt2(361532)
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General description
Rabbit polyclonal anti-Sirt2 antibody recognizes mouse, rat (37/43 kDa), and human (43 kDa) Sirt2. Detection of the Sirt2 bands by immunoblotting is specifically inhibited with the immunizing peptide.
Sirt2 is an NAD+-dependent tubulin deacetylase. Two Sirt2 isoforms exist as the result of alternative splicing. Sirt2 is overexpressed during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle, indicating that it plays a role in the control of mitotic exit in the cell cycle. Although Sirt2 resides predominantly in the cytoplasm, it becomes enriched in the nucleus during the cell cycle where it is associated with mitotic structures. Sirt2 was found to be an oligodendroglial protein that affects cell differentiation through deacetylating α-tubulin.
Sirtuin 2 (SIRT2) is a cytoplasmic protein, which is part of the sirtuin family of proteins. SIRT2 colocalizes with microtubules. This gene is located on human chromosome 19q13.2.
Immunogen
synthetic peptide corresponding to amino acids 341-352 of mouse Sirt2, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human Sirt2.
Application
Anti-Sirt2 antibody has been used in western blotting and immunofluorescence.
Rabbit polyclonal anti-Sirt2 antibody may be used in several applications including immunoblotting, immunoprecipitation, and immunofluorescence.
Biochem/physiol Actions
Sirtuin 2 (SIRT2) maintains the integrity of the genome. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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SIRT2 is required for efficient reprogramming of mouse embryonic fibroblasts toward pluripotency
Kim A Y, et al.
Cell Death & Disease, 9(9), 893-893 (2018)
SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis
Luthi-Carter R, et al.
Proceedings of the National Academy of Sciences of the USA, 201002924-201002924 (2010)
Sirtuin 2 mutations in human cancers impair its function in genome maintenance
Head P E, et al.
The Journal of biological chemistry, jbc-M116 (2017)
SIRT2 ablation has no effect on tubulin acetylation in brain, cholesterol biosynthesis or the progression of Huntington's disease phenotypes in vivo
Bobrowska A, et al.
Testing, 7(4), e34805-e34805 (2012)
Hongdou Luo et al.
Frontiers in aging neuroscience, 9, 366-366 (2017-12-19)
Sirtuins are a class of histone deacetylases (HDACs) that have been shown to regulate a range of pathophysiological processes such as cellular aging, inflammation, metabolism, and cell proliferation. There are seven mammalian Sirtuins (SIRT1-7) that play important roles in stress
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