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S7899

Sigma-Aldrich

Sodium acetate buffer solution

pH 5.2±0.1 (25 °C), for molecular biology, 3 M, 0.2 μm filtered

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Synonym(s):
Sodium acetate buffer 3M
CAS Number:
126-96-5
Beilstein:
3786729
MDL number:
MFCD00137248
UNSPSC Code:
12352200
eCl@ss:
32129211
PubChem Substance ID:
24899767
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

3 M

pH

5.2±0.1 (25 °C)

suitability

suitable for DNA precipitation

storage temp.

room temp

SMILES string

[Na+].CC(O)=O.CC([O-])=O

InChI

1S/2C2H4O2.Na/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+1/p-1

InChI key

BHZOKUMUHVTPBX-UHFFFAOYSA-M

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General description

Sigma′s Sodium Acetate Buffer is a 3M solution prepared with 18 megaohm water and filter-sterilized.

Application

Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay and in vitro enzyme inhibition assay.

Hazard Classifications

Eye Dam. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Tomas Erban et al.
Scientific reports, 7(1), 5084-5084 (2017-07-13)
Honeybee (Apis mellifera L.) workers act as passive vectors of Paenibacillus larvae spores, which cause the quarantine disease American foulbrood (AFB). We assessed the relative proportions of P. larvae within the honeybee microbiome using metabarcoding analysis of the 16 S rRNA
Sena Bluemel et al.
Gut microbes, 11(3), 265-275 (2019-04-16)
Alcohol-induced liver disease is closely related to translocation of bacterial products and bacteria from the intestine to the liver. However, it is not known whether bacterial translocation to the liver depends on certain intestinal microbiota changes that would predispose bacteria
Nadin Rohland et al.
Nature protocols, 13(11), 2447-2461 (2018-10-17)
DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and present only in minute amounts. Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short (≥35 bp) or even ultrashort (≥25
Tong Ihn Lee et al.
Nature protocols, 1(2), 729-748 (2007-04-05)
Genome-wide location analysis, also known as ChIP-Chip, combines chromatin immunoprecipitation and DNA microarray analysis to identify protein-DNA interactions that occur in living cells. Protein-DNA interactions are captured in vivo by chemical crosslinking. Cell lysis, DNA fragmentation and immunoaffinity purification of
Jaspreet Kaur et al.
Antimicrobial agents and chemotherapy, 55(2), 659-666 (2010-12-01)
In this study we utilized the concept of rational drug design to identify novel compounds with optimal selectivity, efficacy and safety, which would bind to the target enzyme pteridine reductase 1 (PTR1) in Leishmania parasites. Twelve compounds afforded from Baylis-Hillman
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Sodium acetate buffer solution for molecular biology

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