S7320
Anti-Spred-2 antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-Sprouty Protein with EVH-1 Domain 2
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~48 kDa
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using PC-12 cell lines
microarray: suitable
western blot: 1-2 μg/mL using cell extracts of HepG2
western blot: 2-4 μg/mL using mouse liver extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SPRED2(200734)
mouse ... Spred2(114716)
rat ... Spred2(305539)
General description
Anti-Spred-2 is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acids of mouse Spred-2, conjugated to KLH via an N terminal added cysteine residue. Spred-2, also known as sprouty protein with EVH-1 domain 2 related sequence, was isolated by similarity to Spred-1, which in turn was isolated from an osteoclast complementary DNA library by a two-hybrid system using c-Kit and c-Fms tyrosine kinase domains as bait. These proteins contain a carboxy-terminal cysteine-rich domain related to sprouty (the SPR domain), and an amino-terminal Ena/vasodilator stimulated phosphoprotein (VASP) homology (EVH-1) domain. This gene is mapped to human chromosome 2p14.
Spred proteins mainly function as inhibitors of the ERK and MAPK signaling cascades. Phosphorylation and Cbl-dependent ubiquitination regulate Spred-2 expression levels. Spred2 negatively modulates the hypothalamic-pituitary-adrenal axis . Anti-Spred-2 antibody is specific for human, rat, and mouse Spred-2 (approx. 48 kDa). Staining of the Spred-2 band in immunoblotting is specifically inhibited by the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acids 280-297 of mouse Spred 2. The sequence is conserved in rat and differs from human by three amino acids.
Application
Anti-Spred-2 antibody produced in rabbit has been used in western blotting and indirect immunofluorescence.
Biochem/physiol Actions
Spred inhibits growth factor mediated activation of MAP kinase. Spred inhibits the activation of MAP kinase probably by mechanisms such as suppression of phosphorylation and activation of Raf. Spred-2 functions as a negative regulator of AGM (aorta-gonad-mesonephros) hematopoiesis by inhibiting hematopoietic cytokine signaling. Spred proteins are also regulators of Rho-mediated cell motility.
Physical form
Solution in 0.01 M phoshate buffered saline, pH 7.4, 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
The VASP-Spred-sprouty domain puzzle
Bundschu K, et al.
The Journal of Biological Chemistry, 281(48), 36477-36481 (2006)
MicroRNA-31 initiates lung tumorigenesis and promotes mutant KRAS-driven lung cancer
Edmonds MD, et al.
The Journal of Clinical Investigation, 126(1), 349-364 (2016)
Genetic variants associated with rheumatoid arthritis patients and serotypes in European populations
Ruiz LN, et al.
Clinical and Experimental Rheumatology, 34(2), 236-241 (2016)
Peter Lock et al.
Biochemical and biophysical research communications, 351(4), 1018-1023 (2006-11-11)
Spred proteins modulate growth factor receptor signaling by inhibiting the Ras-MAPK cascade. Here, we show that Spred-1, Spred-2, and Spred-3 are ubiquitinated in HEK293T cells stimulated with epidermal growth factor (EGF) or pervanadate. Spred-2 tyrosines Y228 and/or Y231 in the
Carlota A García-Domínguez et al.
PloS one, 6(2), e16787-e16787 (2011-03-03)
Sprouty and Spred proteins have been widely implicated in the negative regulation of the fibroblast growth factor receptor-extracellular regulated kinase (ERK) pathway. In considering the functional role of these proteins, we explored their effects on ERK activation induced by cyclopentenone
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