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Quality Level
form
suspension (20% aqueous ethanol)
technique(s)
protein array: suitable
matrix
cross-linked agarose
particle size
24-44 μm
pore size
3-70 kDa MW range (proteins)
500-30,000 Da MW range (dextrans)
operating pH range
1-14(short term)
3-12(long term)
storage temp.
2-8°C
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General description
Combines the high selectivity of a Sephadex matrix with the chemical and physical stability of crosslinked agarose.
Application
Superdex® is used for protein chromatography, gel filtration chromatography, gel filtration media, resins and separation media. Superdex® has been used for the purification and characterization of a novel laccase from the edible mushroom Hericium coralloides. Superdex® has also been used for the purification and mechanism exploration of a potential human hepatocellular carcinoma inhibitor from Bauhinia purpurea L. seeds.
Optimized for high resolution preparative separations by size exclusion chromatography. Recommended fractionation ranges are listed for globular proteins and dextrans.
Legal Information
Superdex is a registered trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
WGK
WGK 3
Flash Point(F)
104.0 °F
Flash Point(C)
40 °C
Regulatory Information
新产品
Certificates of Analysis (COA)
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M Belew et al.
Journal of chromatography. A, 679(1), 67-83 (1994-09-09)
Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), produced as inclusion bodies in genetically transformed Escherichia coli cells was purified to homogeneity by a three-step chromatographic procedure involving hydrophobic interaction, ion exchange and gel filtration. Each purification step is reproducible and well
Toshiro Matsunaga et al.
Plant physiology, 134(1), 339-351 (2003-12-13)
Borate ester cross-linking of the cell wall pectic polysaccharide rhamnogalacturonan II (RG-II) is required for the growth and development of angiosperms and gymnosperms. Here, we report that the amounts of borate cross-linked RG-II present in the sporophyte primary walls of
Cornelia Steinhauer et al.
Proteomics, 6(15), 4227-4234 (2006-07-11)
Antibody-based microarray is a novel technology with great promise in biomedicine that will provide unique means to perform global proteome analysis. In the process of designing the high-density antibody microarrays required, several critical key issues have been identified that remain
S Gibert et al.
Journal of chromatography. B, Biomedical sciences and applications, 737(1-2), 143-150 (2000-02-19)
A kinesin gene has been cloned by RT-PCR (reverse transcription polymerase chain reaction) from Trypanosoma brucei and the corresponding protein overexpressed as a recombinant His-tag (histidine-tag) kinesin in E. coli in order to study its biochemical properties and to determine
Xindu Geng et al.
Journal of biotechnology, 113(1-3), 137-149 (2004-09-24)
A new technology for renaturation with simultaneous purification of the recombinant human interferon-gamma (rhIFN-gamma) in downstream of biotechnology is presented. The strategies to develop the new technology in industry scale were suggested. Based on chemical equilibrium and molecular interactions, the
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