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General description
The DNA-blocked “Staph-Seq” cells are ideally suited for studying recruitment of low abundance transcription factors (TF) in genome wide location analysis experiments such as ChIP-chip and ChIP-Seq. Sequencing quality modified Staph A (Staph-Seq) is achieved by employing a proprietary DNA-blocking technology to prevent Staph A DNA contamination of next-generation sequencing data in ChIP-Seq experiments. The high surface-density of endogenous protein A on the cell wall of Staph A serves as an excellent matrix to pull-down rare TF-associated chromatin complexes.
Learn more about ChIP kits and protocol.
Learn more about ChIP kits and protocol.
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Sepharose is a trademark of Cytiva
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WGK
WGK 3
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Not applicable
Flash Point(C)
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Epigenome-wide analysis of DNA methylation in lung tissue shows concordance with blood studies and identifies tobacco smoke-inducible enhancers
Theresa Ryan Stueve
Human Molecular Genetics (2017)
Theresa Ryan Stueve et al.
Human molecular genetics, 26(15), 3014-3027 (2017-09-01)
Smoking-associated DNA hypomethylation has been observed in blood cells and linked to lung cancer risk. However, its cause and mechanistic relationship to lung cancer remain unclear. We studied the association between tobacco smoking and epigenome-wide methylation in non-tumor lung (NTL)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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