Skip to Content
Merck
CN
All Photos(2)

Key Documents

View All Documentation

S6322

Sigma-Aldrich

Sigma Adjuvant System®

oil

Synonym(s):

Adjuvant System, Sigma Adjuvant

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

form

oil

Quality Level

300

storage temp.

2-8°C

Application

Adjuvanticity Assay: EIA titer for test mice is at least 2X greater that that of control mice.

Biochem/physiol Actions

Sigma Adjuvant System is a stable oil-in-water emulsion that may be used as an alternative to the classical Freund′s water-in-oil emulsions. It is derived from bacterial and mycobacterial cell wall components that provide potent stimulus to the immune system.

Physical form

Each vial contains 0.5 mg Monophosphoryl Lipid A (detoxified endotoxin) from Salmonella minnesota and 0.5 mg synthetic Trehalose Dicorynomycolate in 2% oil (squalene)-Tween® 80-water.

Legal Information

Sigma Adjuvant System is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

related product

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H304

Precautionary Statements

P301 + P310 - P331 - P405 - P501

Hazard Classifications

Asp. Tox. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000329924
0000235360
0000235360
0000329924
0000245077

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. Is the Sigma Adjuvant System®, Product No S6322, the same as the RIBI adjuvant?

    Yes, the Sigma Adjuvant System®, Product No S6322, is also called RIBI adjuvant. 

  2. What is used to solubilize Product No. S6322, Sigma Adjuvant System® reagent?

    The Sigma Adjuvant System® reagent (Product No. S6322) can be solubilized in a sterile saline solution containing the desired amount of antigen to a final volume of 2 ml.  For more details, please see the S6322 Product Information Sheet.

  3. Can you reconstitute and store the Sigma Adjuvant System® reagent (Product No. S6322) if not using the entire vial right away?

    If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen.  This emulsion can then be stored at 2-8 °C for up to 60 days.  Do Not Freeze. To use, mix aliquots 1:1 with antigen in saline. Prior to inoculation, warm the vial to 37 °C and vortex briefly.  This and other information can be found on the Sigma Adjuvant System® reagent (Product No. S6322) Product Information Sheet.

  4. When using the Sigma Adjuvant System®, Product No. S6322, how is the antigenic response boosted?

    With the Sigma Adjuvant System® (Product No. S6322) with mice, rabbits, rats and guinea pigs; boost on day 21 or 28, bleed 10 - 14 days after booster injection. Boost every 3 - 4 weeks thereafter. For goats; boost every four weeks. Use the same protocol for each injection.  For more information please see the Product Information Sheet for Product No. S6322.

  5. When using the Sigma Adjuvant System® reagent, Product No. S6322, how do the components MPL and TDM work as an adjuvant?

    MPL (monophosphoryl lipid A) will bind to CD14 on the cell surface similar to LPS.  TDM (trehalose dicorynomycolate) is an analog of one of the components of the tubercle bacillus. Both of these components act like a superantigen and activate the immune system nonspecifically.  This allows for a greater response to the immunogen delivered in this adjuvant.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. Has Sigma Adjuvant System®, Product S6322, been used to induce EAE (Experimental autoimmune encephalomyelitis)?

    Sigma Adjuvant System®, Product S6322 is a replacement for MPT+TDM adjuvant (Ribi, Product M6536) that was discontinued by the supplier Corixa.  Although we have not tested Product S6322 for induction of Experimental autoimmune encephalomyelitis (EAE), the following reference cites the use of MBP in Ribi adjuvant for induction of acute EAE.The Journal of ImmunologyOctober 15, 2005, 175(8): 5077-5086

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Christina Schellenbacher et al.
Journal of virology, 83(19), 10085-10095 (2009-07-31)
The amino (N) terminus of the human papillomavirus (HPV) minor capsid protein L2 can induce low-titer, cross-neutralizing antibodies. The aim of this study was to improve immunogenicity of L2 peptides by surface display on highly ordered, self-assembled virus-like particles (VLP)
Nadja Thönes et al.
Journal of virology, 82(11), 5472-5485 (2008-04-04)
Capsomeres are considered to be an alternative to viruslike particle (VLP)-based vaccines as they can be produced in prokaryotic expression systems. So far, no detailed side-by-side comparison of VLPs and capsomeres has been performed. In the present study, we immunized
Avi-Hai Hovav et al.
Infection and immunity, 73(1), 250-257 (2004-12-25)
In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-gamma) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant
Déirdre B Ní Eidhin et al.
FEMS immunology and medical microbiology, 52(2), 207-218 (2007-12-21)
Clostridium difficile is the leading cause of infectious antibiotic-associated diarrhoea, particularly among the elderly. Its surface-layer protein (SLP) was tested as a vaccine component in a series of immunization and challenge experiments with Golden Syrian hamsters, combined with different systemic
Gabriel Ozorowski et al.
Journal of virology, 92(13) (2018-04-20)
Native-like, soluble, recombinant SOSIP trimers of various designs and based on several env genes of human immunodeficiency virus type 1 (HIV-1) are being tested as immunogens in different animal models. These experiments almost always involve coformulating the trimers with an
View All Related Papers

Articles

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Related Content

Antibody Support Reagents

We offer the highest quality reagents to support your antibody workflows including adjuvants, antibody blocking reagents and substrates, cell capture imaging reagents, antibody conjugation and labeling kits, and more.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service