S4195
β-Secretase human
recombinant, expressed in HEK 293 cells (C-terminal FLAG tagged), extracellular domain, ≥10,000 units/mg protein, ≥90% (SDS-PAGE), buffered aqueous solution
Synonym(s):
Asp-2, BACE1, Memapsin-2
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About This Item
recombinant
expressed in HEK 293 cells (C-terminal FLAG tagged)
Quality Level
Assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥10,000 units/mg protein
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... BACE1(23621)
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Related Categories
Application
Human β-secretase or BACE1 has been used to study the binding reaction between amyloid precursor protein and β-secretase. It has also been used in BACE1 assay.
Biochem/physiol Actions
BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s, therefore BACE1 is a target for inhibitor drug discovery.
BACE1 (β-site APP-cleaving enzyme 1) is a transmembrane aspartyl protease responsible for cleaving the amyloid precursor protein (APP). BACE1-mediated cleavage of amyloid precursor protein (APP) is the first step during the generation of pathogenic amyloid-β peptides, hence making it the major drug target for Alzheimer′s disease (AD). Increased levels of enzymatic activity of BACE1 was found in cerebrospinal fluid from patients having both mild cognitive impairment (MCI) and AD, thereby suggesting the importance of BACE1 enzymatic activity in conversion of MCI to AD.
Unit Definition
One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acetyl-[Asn670, Leu671]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-(2,4-dinitrophenyl)-Lys-Arg-Arg amide substrate per 1 minute at pH 4.5 at 37 °C.
Physical form
Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Current neurovascular research (2013)
Acceleration of deposition of peptide on ultrasonically formed nucleus studied by wireless quartz-crystal-microbalance biosensor
Hirotsugu Ogi
Biosensors And Bioelectronics (2013)
Roland Nicsanu et al.
Journal of Alzheimer's disease : JAD, 87(1), 433-441 (2022-03-12)
Beta-site APP cleaving enzyme 1 (BACE1) is the rate-limiting enzyme in amyloid-β (Aβ) plaques formation. BACE1 activity is increased in brains of patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) and plasma levels of BACE1 appears to reflect
Platelet-Derived Secreted Amyloid-Precursor Protein-? as a Marker for Diagnosing Alzheimer?s Disease
Josef M and Christian H
Current neurovascular research (2013)
Carlo Cervellati et al.
GeroScience, 42(1), 159-167 (2019-11-21)
Beta-secretase (BACE1) is a key enzyme in the formation of amyloid-β; its activity/concentration is increased in brain and cerebrospinal fluid of patients with late-onset Alzheimer's disease (LOAD). Since BACE1 was found also in blood, we evaluated its potential as peripheral
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