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S400HR

Sephacryl® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
MFCD00165824

Key Documents

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Product Name

Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

Quality Level

200

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Application

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.

General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S400HR-750ML′s updated product is GE17-0609-01

Legal Information

Sephacryl is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKCW2518
MKCN9954
MKCJ0342
MKCG9384
MKCF7412

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E Käslan et al.
The Journal of biological chemistry, 269(19), 14103-14110 (1994-05-13)
During purification of a strand exchange activity from Schizosaccharomyces pombe using the three-strand reaction of double-stranded linear and circular single-stranded DNA, we identified p190/210 as an activity that stimulated the strand exchange activity of p140exo2 by about 10-fold. The accompanying
Hao Wang et al.
MethodsX, 7, 100816-100816 (2020-03-21)
Hydrogen peroxide (H2O2) is an important signal molecule produced in animal and plant cells. The balance of H2O2 between the intra- and extracellular space is regulated by integral membrane proteins, which thereby modulate signaling. Several methods have been established to
Enhancement of drug delivery to bone: Characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide
Nishioka T, et al.
Molecular Genetics and Metabolism, 88(3), 244-255 (2006)
Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
Sinu P John et al.
Journal of virology, 81(17), 8967-8976 (2007-06-15)
The Ebola virus (EBOV) genome encodes for several proteins that are necessary and sufficient for replication and transcription of the viral RNAs in vitro; NP, VP30, VP35, and L. VP30 acts in trans with an RNA secondary structure upstream of
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