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S1451

Sigma-Aldrich

Anti-Sox2 antibody, Mouse monoclonal

clone SOX2-6, purified from hybridoma cell culture

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Synonym(s):
Anti-ANOP3, Anti-MCOPS3, Anti-SRY (sex determining region Y)-box 2, Anti-SRY-related HMG-box gene 2, Anti-Sex-determining region Y-box 2
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SOX2-6, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using whole extract of human NT2 cells

isotype

IgG1

UniProt accession no.

P48431

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SOX2(6657)
mouse ... Sox2(20674)
rat ... Sox2(499593)

General description

Monoclonal Anti-SOX2 (mouse IgG1 isotype) is derived from the hybridoma SOX2-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.
SOX2 (sex-determining region Y-box 2) is a transcription factor that is made of 317 amino acid. It has a HMG (high mobility group box) domain and is a critical transcription regulator of normal stem cell. It is located on human chromosome 3q26.3.

Application

Anti-Sox2 antibody has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
SOX2 (sex-determining region Y-box 2) down-regulation reduces the stem cell count, that affects the breast cancer cells in initiating tumor progression. It participates in chemoresistance and regular lung cancer therapies. In mouse, SOX2 plays an important role in branching morphogenesis and regulating lung epithelial cell differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

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Flash Point(C)

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Regulatory Information

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Differential expression of ID4 and its association with TP53 mutation, SOX2, SOX4 and OCT-4 expression levels
Galatro TF, et al.
PLoS ONE, 8(4) (2013)
Thais Fernanda de Almeida Galatro et al.
PloS one, 8(4), e61605-e61605 (2013-04-25)
Inhibitor of DNA Binding 4 (ID4) is a member of the helix-loop-helix ID family of transcription factors, mostly present in the central nervous system during embryonic development, that has been associated with TP53 mutation and activation of SOX2. Along with
CtBP-interacting BTB zinc finger protein (CIBZ) promotes proliferation and G1/S transition in embryonic stem cells via Nanog
Nishii T, et al.
The Journal of Biological Chemistry, 287(15), 12417-12424 (2012)
CIBZ Regulates Mesodermal and Cardiac Differentiation of by Suppressing T and Mesp1 Expression in Mouse Embryonic Stem Cells
Kotoku T, et al.
Scientific Reports, 6 (2016)
Mutations in SOX2 cause anophthalmia
Fantes J, et al.
Nature Genetics, 33(4), 462-462 (2003)
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