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S1402

Sigma-Aldrich

Sulforhodamine B sodium salt

powder, BioReagent, suitable for cell culture

Synonym(s):

Kiton Red 620

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About This Item

Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein:
3886008
EC Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

Quality Level

product line

BioReagent

form

powder

composition

Dye content, ~75%

technique(s)

cell culture | mammalian: suitable

solubility

methanol: 1 mg/mL

fluorescence

λex 565 nm; λem 586 nm in H2O

SMILES string

[Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1

InChI

1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1

InChI key

SXQCTESRRZBPHJ-UHFFFAOYSA-M

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General description

Sulforhodamine B is a rhodamine derived aminoxanthene dye. It contains two sulfonic acids groups which separate in alkaline condition.

Application

Sulforhodamine B sodium salt has been used:
  • for relative total cell mass quantification
  • to investigate the viscosity of coacervates
  • as a molecular rotor for microviscosity determination in aerosol droplets

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.

related product

Product No.
Description
Pricing

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bioconjugate Techniques, 317-317 (1996)
Issues in Pharmacology, Pharmacy, Drug Research, and Drug Innovation (2012)
Luca Giordano et al.
American journal of respiratory cell and molecular biology, 60(5), 515-522 (2018-10-20)
Cigarette smoke (CS) exposure is the predominant risk factor for the development of chronic obstructive pulmonary disease (COPD) and the third leading cause of death worldwide. We aimed to elucidate whether mitochondrial respiratory inhibition and oxidative stress are triggers in
Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency
Pereira SL, et al.
PLoS ONE, 8(12), e82095-e82095 (2013)
Fluorescent lifetime imaging of atmospheric aerosols: a direct probe of aerosol viscosity
Faraday Discussions, 165, 343-356 (2013)

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