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Safety Information

S100HR

Sigma-Aldrich

Sephacryl®

100-HR, MW range 1000-100,000 Da (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
23151817
NACRES:
NA.56

Quality Level

200

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)
25-75 μm (wet)

pore size

1000-100,000 Da MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Application

Sephacryl® has been used:
  • to develop a novel high-throughput screening assay for sickle cell disease drug discovery
  • to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

WGK

WGK 1

Flash Point(F)

closed cup

Flash Point(C)

closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

Certificates of Analysis (COA)

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Marc A Benson et al.
Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt
Diederik Johannes Opperman et al.
Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
Matthew Lord et al.
The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
B H Kang et al.
Archives of biochemistry and biophysics, 263(2), 387-393 (1988-06-01)
A simple procedure using ammonium sulfate to fractionate water-soluble cytochromes c553 and c550 on Sephacryl S-200 gel is described. The usefulness of this procedure has been studied using the crude extracts of mesophilic cyanobacteria. It was found that almost all
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