S0698
Monoclonal Anti-SmB antibody produced in mouse
~1 mg/mL, clone 12F5, purified immunoglobulin, buffered aqueous solution
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Synonym(s):
Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12F5, monoclonal
form
buffered aqueous solution
mol wt
antigen ~25 kDa
species reactivity
human, mouse, canine
concentration
~1 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SNRPB(6628)
mouse ... Snrpb(20638)
General description
Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.
SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.
Immunogen
SmB recombinant protein.
Application
- Monoclonal Anti-SmB antibody produced in mouse has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein
Skrajna A, et al.
RNA, 23(6), 938-951 (2017)
Smed-SmB, a member of the LSm protein superfamily, is essential for chromatoid body organization and planarian stem cell proliferation
Fernandez-TE, et al.
Development, 137(7), 1055-1065 (2010)
Functional genomics analyses of RNA-binding proteins reveal the splicing regulator SNRPB as an oncogenic candidate in glioblastoma
Correa BR, et al.
Genome Biology, 17(1) (2016)
Concerted regulation and molecular evolution of the duplicated SNRPB'/B and SNRPN loci
Gray TA, et al.
Nucleic Acids Research, 27(23), 4577-4584 (1999)
Allison E Drew et al.
Scientific reports, 7(1), 17993-17993 (2017-12-23)
CARM1 is an arginine methyltransferase with diverse histone and non-histone substrates implicated in the regulation of cellular processes including transcriptional co-activation and RNA processing. CARM1 overexpression has been reported in multiple cancer types and has been shown to modulate oncogenic
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