RNB300
GenElute™ Single Cell RNA Purification Kit
Synonym(s):
RNA extraction kit for NGS, RNA extraction kit for qPCR, RNA isolation kit for NGS, RNA isolation kit for qPCR, RNA purification kit for NGS, RNA purification kit for qPCR, single cell RNA extraction kit, single cell RNA isolation kit, single cell RNA kit, single cell total RNA extraction kit, single cell total RNA isolation kit, single cell total RNA purification kit
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About This Item
UNSPSC Code:
41105600
NACRES:
NA.52
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shipped in
ambient
storage temp.
room temp
General description
Sigma′s GenElute™ Single Cell RNA Purification Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). The kit can recover RNA from as little as a single cell to 2 x 10^5 cells. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
- Fast and easy processing using rapid micro spin-column format
- Small elution volume of 8 μL
- Isolate total RNA, from large rRNA down to microRNA (miRNA) without compromising total yield
- No phenol or chloroform extractions
- Isolate high quality total RNA from a variety of sources
- RNA can be isolated and detected from as little as a single animal cell
Application
- Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
- RT-PCR for both large mRNA and small RNA including miRNA
- Expression profiling
- Next Generation Sequencing for RNA and miRNA
- microRNA cloning and amplification
- Northern blotting
- RNase protection
- Primer extension
- miRNA array
- RNA-Seq
- Nanostring
- Fluidigm
- Droplet/Digital PCR
Features and Benefits
- Ideal for small inputs - Total RNA can be isolated from small input amounts of cultured animal cells, sorted cells, and microdissected samples including laser-capture microdissection (LCM). RNA has been isolated from as little as a single cell.
- Smal elution volume - Elute total RNA in as little as 8 μL of Elution Buffer
- No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
- Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) without compromising yield
- Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
Unit Definition
Preps
Other Notes
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 2
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