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RES3098T-B7

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TRIS hydrochloride

Synonym(s):

Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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About This Item

Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
1185-53-1
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
214-684-5
MDL number:
MFCD00012590
UNSPSC Code:
12352200
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals, tested

useful pH range

7.0-9.0

pKa (25 °C)

8.1

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Application

Tris Hydrochloride is a commonly used biological buffer which is also referred to as Trizma HCl. Tris as a buffer has very broad usage in biological systems such as diagnostics, downstream purification and cell culture. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.

Tris HCl s used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.

Packaging

Product is available in the following package sizes:
RES3098T-B701X: 100g container
RES3098T-B703X: 5kg container
RES3098T-B705X: 25kg container
RES3098T-B706X: 50kg container

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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