RAB1089
Human TNF-alpha ELISA Kit (for Lysates)
for cell and tissue lysate samples
Synonym(s):
TNF-Alpha
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
input
sample type tissue lysate
sample type cell lysate
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/ml
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TNF(7124)
General description
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4
Tumor necrosis factor-α (TNF-α) is encoded by the gene mapped to human chromosome 6p21.3, within the major histocompatibility complex (MHC) class III region. The encoded protein belongs to the TNF superfamily.
Application
Please refer to the attached Protocolfor details.
Human tumor necrosis factor-alpha (TNF-α) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF-α in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).
Human tumor necrosis factor-alpha (TNF-α) ELISA Kit has been used in enzyme-linked immunosorbent assay (ELISA) to quantify TNF-α in supernates of human acute lung infection model (ALIM), human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC-1).
Biochem/physiol Actions
Tumor necrosis factor-α (TNF-α) is a key immunomodulator and proinflammatory cytokine. It is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Mutation in the gene increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma. In addition, polymorphism in the gene leads to obsessive-compulsive disorder (OCD) and osteoarthritis (OA).
Other Notes
A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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