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Safety Information

RAB0480

Sigma-Aldrich

Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates

Synonym(s):

Cachectin, TNF-alpha, Tumor necrosis factor alpha

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50 μG
CN¥2,102.30

CN¥2,102.30

Estimated to ship onJuly 04, 2025Details

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50 μG
CN¥2,102.30

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

CN¥2,102.30

Estimated to ship onJuly 04, 2025Details

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Tnf(24835)

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manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

solution

form

solution

form

solution

form

solution

color

colorless

color

colorless

color

colorless

color

colorless

packaging

pkg of 50 μg (in 200 μl)

packaging

pkg of 50 μg (250 μg/ml)

packaging

pkg of 50 μg (in 200 μl)

packaging

pkg of 50 μg (in 200 μl)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.

Immunogen

Recombinant Rat TNF-α

Application

For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.[1][2]

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats.[3] Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.[4]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)
  • RABLYSIS12X Cell Lysis Buffer (Item J)
  • RABSTOP3ELISA Stop Solution (Item I)
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0602D0715
    0424K0715
    0404K0715
    0320K0715
    1114J0715

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Tumor necrosis factor-alpha: a possible priming agent for the polymorphonuclear leukocyte-reduced nicotinamide-adenine dinucleotide phosphate oxidase in hypertension.
    Mazor R
    Hypertension, 55(2), 353-362 (2010)
    Hanaa Mohamed Khalaf et al.
    Clinical and experimental hepatology, 5(1), 65-74 (2019-03-28)
    Non-alcoholic fatty liver disease (NAFLD) is a challenging health problem. Hyperuricemia is a key player in the pathogenesis of NAFLD. This study investigated the effect of allopurinol (uric acid synthesis inhibitor) in combination with metformin and vitamin E in prevention
    Meijuan Chen et al.
    Gastroenterology research and practice, 2019, 8723460-8723460 (2019-10-01)
    Phosphatidylcholine (PC) is the major surface-active phospholipid and creates a hydrophobic nature to the surface. It has been reported to reverse the progression of liver fibrosis and to improve liver function. The aim of the present study was to evaluate
    Diosmin Modulates the NF-kB Signal Transduction Pathways and Downregulation of Various Oxidative Stress Markers in Alloxan-Induced Diabetic Nephropathy.
    Ahmed S
    Inflammation, 39(5), 1783-1797 (2016)
    Vu Thi Thu et al.
    The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 20(3), 305-314 (2016-05-11)
    Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to
    View All Related Papers

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